Laboratory of Transmissible Diseases and Biological Active Substances, University of Monastir, Monastir, Tunisia.
J Med Virol. 2012 Nov;84(11):1818-24. doi: 10.1002/jmv.23385.
The polyomavirus JC (JCPyV) is a ubiquitous virus in humans, causing progressive multifocal leukoencephalopathy, a fatal demyelinating disease. JCPyV propagates in the adult kidney and excretes its progeny in urine, from which its DNA can be recovered readily. JCPyV isolates worldwide can be classified into 14 subtypes or genotypes, each associated with a specific geographical region. The European genotypes EU-a-b-c are spread throughout Europe and Mediterranean areas. The major African genotype Af2 is spread not only throughout Africa but also in West and South Asia. A minor African genotype (Af1) occurs in Central and West Africa. Partially overlapping domains in Asia were occupied by various genotypes (e.g., B1-a, -b, -d, B2, CY, MY, and SC). To characterize the subtypes of JCPyV prevalent in Tunisia, the presence of the virus was investigated by real-time PCR in urine samples from 98 renal transplant recipients. For subtype identification, a 610 bp typing region of the JCPyV genome was amplified from each urine sample, and its DNA sequence was determined. In the patients studied, the major African subtype Af2 was the predominant (62.5%), followed by the European subtype EU (33.5%). Only one case clustering with the Asian genotype SC (4%) was identified. The presence of the European subtype with high prevalence in this population suggests that the epidemiological distribution of JCPyV virus sequences in North Africa is related partially to the epidemiological data in Europe.
多瘤病毒 JC（JCPyV）是一种普遍存在于人类中的病毒，可引起进行性多灶性白质脑病，这是一种致命的脱髓鞘疾病。JCPyV 在成人肾脏中繁殖，并将其后代排泄在尿液中，从尿液中可以很容易地回收其 DNA。世界各地的 JCPyV 分离株可分为 14 种亚型或基因型，每种亚型或基因型都与特定的地理区域相关。欧洲基因型 EU-a-b-c 分布于整个欧洲和地中海地区。主要的非洲基因型 Af2 不仅分布于非洲，还分布于西亚和南亚。次要的非洲基因型（Af1）存在于中非和西非。亚洲部分重叠的区域则由各种基因型（如 B1-a、-b、-d、B2、CY、MY 和 SC）占据。为了描述突尼斯流行的 JCPyV 亚型，通过实时 PCR 检测了 98 名肾移植受者尿液样本中的病毒。为了进行亚型鉴定，从每个尿液样本中扩增 JCPyV 基因组的 610bp 分型区域，并确定其 DNA 序列。在所研究的患者中，主要的非洲亚型 Af2 是最主要的（62.5%），其次是欧洲亚型 EU（33.5%）。仅鉴定出 1 例与亚洲基因型 SC（4%）聚类的病例。该人群中欧洲亚型的高流行率表明，北非 JCPyV 病毒序列的流行病学分布部分与欧洲的流行病学数据有关。
