• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

NOD2激动剂与TLR2和TLR4激动剂协同促进血管平滑肌细胞中炎性细胞因子的产生。

NOD2 agonist promotes the production of inflammatory cytokines in VSMC in synergy with TLR2 and TLR4 agonists.

作者信息

Sun Jinghua, Ding Yanchun

机构信息

Department of Oncology, The Second Affiliated Hospital of Dalian Medical University, Dalian 116027, China.

出版信息

ScientificWorldJournal. 2012;2012:607157. doi: 10.1100/2012/607157. Epub 2012 Sep 10.

DOI:10.1100/2012/607157
PMID:22997500
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3446649/
Abstract

OBJECTIVE

To investigate the expression of NOD2 in human VSMCs, its role in the production of inflammatory cytokines in VSMC and the possible interaction of NOD2-mediated signaling pathway with those mediated by TLR2 and TLR4.

METHODS

Human coronary artery smooth muscle cells were stimulated with NOD2 agonist MDP alone or in combination with either TLR2 agonist PAM3 or TLR4 agonist LPSs. The mRNA expression of NOD2 and FGF-2 were measured by RT-PCR. The concentration of IL-8 and TNF-α in the culture supernatants was determined by ELISA. VSMC proliferation ability was analyzed by MTT assay.

RESULTS

MDP up regulated the expression of NOD2 mRNA in VSMC in a time-dependent manner, up regulated the expression of FGF-2 mRNA in VSMC, induced the production of IL-8 and TNF-α, and promoted the proliferation of VSMC. Additionally, MDP synergied with LPS and PAM3 to promote the proliferation of VSMC and induce the production of IL-8 and TNF-α.

CONCLUSION

The activation of NOD2-mediated innate immune signaling pathway can increase the proliferation ability of VSMC and induce the production of inflammatory cytokines in VSMC. It is also shown a synergistic effect with TLR2- and TLR4-mediated signaling pathways in this process.

摘要

目的

研究NOD2在人血管平滑肌细胞（VSMCs）中的表达，其在VSMC中炎性细胞因子产生中的作用以及NOD2介导的信号通路与TLR2和TLR4介导的信号通路之间可能的相互作用。

方法

用人冠状动脉平滑肌细胞单独用NOD2激动剂MDP刺激，或与TLR2激动剂PAM3或TLR4激动剂LPSs联合刺激。通过RT-PCR检测NOD2和FGF-2的mRNA表达。用ELISA测定培养上清液中IL-8和TNF-α的浓度。通过MTT法分析VSMC增殖能力。

结果

MDP以时间依赖性方式上调VSMC中NOD2 mRNA的表达，上调VSMC中FGF-2 mRNA的表达，诱导IL-8和TNF-α的产生，并促进VSMC的增殖。此外，MDP与LPS和PAM3协同促进VSMC的增殖并诱导IL-8和TNF-α的产生。

结论

NOD2介导的天然免疫信号通路的激活可增加VSMC的增殖能力并诱导VSMC中炎性细胞因子的产生。在此过程中还显示出与TLR2和TLR4介导的信号通路具有协同作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cac2/3446649/6b14fef971f8/TSWJ2012-607157.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cac2/3446649/a9f0da3184b5/TSWJ2012-607157.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cac2/3446649/9f809d19122a/TSWJ2012-607157.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cac2/3446649/e13f49202c37/TSWJ2012-607157.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cac2/3446649/6b14fef971f8/TSWJ2012-607157.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cac2/3446649/a9f0da3184b5/TSWJ2012-607157.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cac2/3446649/9f809d19122a/TSWJ2012-607157.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cac2/3446649/e13f49202c37/TSWJ2012-607157.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cac2/3446649/6b14fef971f8/TSWJ2012-607157.004.jpg

相似文献

1
NOD2 agonist promotes the production of inflammatory cytokines in VSMC in synergy with TLR2 and TLR4 agonists.NOD2激动剂与TLR2和TLR4激动剂协同促进血管平滑肌细胞中炎性细胞因子的产生。
ScientificWorldJournal. 2012;2012:607157. doi: 10.1100/2012/607157. Epub 2012 Sep 10.
2
Expression of TRAF6 and pro-inflammatory cytokines through activation of TLR2, TLR4, NOD1, and NOD2 in human periodontal ligament fibroblasts.TLR2、TLR4、NOD1 和 NOD2 激活人牙周膜成纤维细胞中 TRAF6 和促炎细胞因子的表达。
Arch Oral Biol. 2011 Oct;56(10):1064-72. doi: 10.1016/j.archoralbio.2011.02.020. Epub 2011 Mar 31.
3
The crosstalk between TLR2 and NOD2 in Aspergillus fumigatus keratitis.TLR2 与 NOD2 在烟曲霉菌角膜炎中的串扰。
Mol Immunol. 2015 Apr;64(2):235-43. doi: 10.1016/j.molimm.2014.11.021. Epub 2014 Dec 27.
4
TLR 2 induces vascular smooth muscle cell migration through cAMP response element-binding protein-mediated interleukin-6 production.TLR2 通过 cAMP 反应元件结合蛋白介导体细胞间白细胞介素 6 的产生而诱导血管平滑肌细胞迁移。
Arterioscler Thromb Vasc Biol. 2012 Nov;32(11):2751-60. doi: 10.1161/ATVBAHA.112.300302. Epub 2012 Sep 20.
5
Muramyl dipeptide enhances osteoclast formation induced by lipopolysaccharide, IL-1 alpha, and TNF-alpha through nucleotide-binding oligomerization domain 2-mediated signaling in osteoblasts.胞壁酰二肽通过成骨细胞中核苷酸结合寡聚化结构域2介导的信号传导增强脂多糖、白细胞介素-1α和肿瘤坏死因子-α诱导的破骨细胞形成。
J Immunol. 2005 Aug 1;175(3):1956-64. doi: 10.4049/jimmunol.175.3.1956.
6
Chronic stimulation of Nod2 mediates tolerance to bacterial products.Nod2的慢性刺激介导对细菌产物的耐受性。
Proc Natl Acad Sci U S A. 2007 Dec 4;104(49):19440-5. doi: 10.1073/pnas.0706097104. Epub 2007 Nov 21.
7
Nod2 and Rip2 contribute to innate immune responses in mouse neutrophils.Nod2和Rip2在小鼠中性粒细胞的天然免疫反应中发挥作用。
Immunology. 2014 Oct;143(2):269-76. doi: 10.1111/imm.12307.
8
Peptidoglycan and muramyl dipeptide from Staphylococcus aureus induce the expression of VEGF-A in human limbal fibroblasts with the participation of TLR2-NFκB and NOD2-EGFR.金黄色葡萄球菌的肽聚糖和 muramyl 二肽通过 TLR2-NFκB 和 NOD2-EGFR 途径诱导人角膜缘成纤维细胞中 VEGF-A 的表达。
Graefes Arch Clin Exp Ophthalmol. 2013 Jan;251(1):53-62. doi: 10.1007/s00417-012-2130-5. Epub 2012 Aug 14.
9
Nod2 downregulates TLR2/1 mediated IL1β gene expression in mouse peritoneal macrophages.Nod2 下调 TLR2/1 介导的小鼠腹腔巨噬细胞中 IL1β 基因的表达。
PLoS One. 2011;6(11):e27828. doi: 10.1371/journal.pone.0027828. Epub 2011 Nov 17.
10
Synergistic effect of Nod1 and Nod2 agonists with toll-like receptor agonists on human dendritic cells to generate interleukin-12 and T helper type 1 cells.Nod1和Nod2激动剂与Toll样受体激动剂对人树突状细胞产生白细胞介素-12和1型辅助性T细胞的协同作用。
Infect Immun. 2005 Dec;73(12):7967-76. doi: 10.1128/IAI.73.12.7967-7976.2005.

引用本文的文献

1
Immune cells and vasa vasorum in the tunica media of atherosclerotic coronary arteries.动脉中层粥样硬化冠状动脉中的免疫细胞和血管周细胞。
Bosn J Basic Med Sci. 2018 Aug 1;18(3):240-245. doi: 10.17305/bjbms.2018.2951.
2
Role of Muramyl Dipeptide in Lipopolysaccharide-Mediated Biological Activity and Osteoclast Activity.肽聚糖二肽在脂多糖介导的生物学活性和破骨细胞活性中的作用。
Anal Cell Pathol (Amst). 2018 Feb 14;2018:8047610. doi: 10.1155/2018/8047610. eCollection 2018.

本文引用的文献

1
Micromanaging vascular smooth muscle cell differentiation and phenotypic modulation.微血管平滑肌细胞分化和表型调节的微观管理。
Arterioscler Thromb Vasc Biol. 2011 Nov;31(11):2370-7. doi: 10.1161/ATVBAHA.111.226670.
2
Inhibition of pattern recognition receptor-mediated inflammation by bioactive phytochemicals.生物活性植物化学物质抑制模式识别受体介导的炎症反应。
Nutr Rev. 2011 Jun;69(6):310-20. doi: 10.1111/j.1753-4887.2011.00394.x.
3
Role of toll-like receptors in cardiovascular diseases. toll 样受体在心血管疾病中的作用。
Clin Sci (Lond). 2011 Jul;121(1):1-10. doi: 10.1042/CS20100539.
4
Nod-like receptors: sentinels at host membranes.核苷酸结合寡聚化结构域样受体：宿主细胞膜上的哨兵。
Curr Opin Immunol. 2010 Aug;22(4):428-34. doi: 10.1016/j.coi.2010.04.010. Epub 2010 Jun 3.
5
The role of pattern-recognition receptors in innate immunity: update on Toll-like receptors.模式识别受体在天然免疫中的作用：Toll 样受体更新。
Nat Immunol. 2010 May;11(5):373-84. doi: 10.1038/ni.1863. Epub 2010 Apr 20.
6
Human heat shock protein 60 stimulates vascular smooth muscle cell proliferation through Toll-like receptors 2 and 4.人类热休克蛋白60通过Toll样受体2和4刺激血管平滑肌细胞增殖。
Microbes Infect. 2006 Jun;8(7):1859-65. doi: 10.1016/j.micinf.2006.02.024. Epub 2006 May 3.
7
Platelet-derived growth factor-BB transactivates the fibroblast growth factor receptor to induce proliferation in human smooth muscle cells.血小板衍生生长因子-BB通过反式激活成纤维细胞生长因子受体来诱导人平滑肌细胞增殖。
Trends Cardiovasc Med. 2006 Jan;16(1):25-8. doi: 10.1016/j.tcm.2005.11.003.
8
Synergistic stimulation of human monocytes and dendritic cells by Toll-like receptor 4 and NOD1- and NOD2-activating agonists.Toll样受体4以及NOD1和NOD2激活激动剂对人单核细胞和树突状细胞的协同刺激作用
Eur J Immunol. 2005 Aug;35(8):2459-70. doi: 10.1002/eji.200526286.
9
NOD-LRR proteins: role in host-microbial interactions and inflammatory disease.NOD样受体蛋白：在宿主-微生物相互作用及炎症性疾病中的作用
Annu Rev Biochem. 2005;74:355-83. doi: 10.1146/annurev.biochem.74.082803.133347.
10
Agonists of toll-like receptors 2 and 4 activate airway smooth muscle via mononuclear leukocytes.
Am J Respir Crit Care Med. 2005 Apr 15;171(8):814-22. doi: 10.1164/rccm.200403-406OC. Epub 2005 Jan 18.