Wang Yinjie, Zhao Ruizhi, Lu Chuanjian
Second Clinical College, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China.
Zhongguo Zhong Yao Za Zhi. 2012 Jun;37(12):1859-61.
To establish a SPE-HPLC method for analyzing astilbin in rats serum and explore the effects of Yinxieling (YXL) prescription and Smilacis Glabrae Rhizoma on the pharmacokinetic characteristics of effective components.
Male Sprague-dawley rats were administrated YXL and Smilacis Glabrae Rhizoma respectively. At different time points, serum concentration of astilbin was extracted by Solid Phase Extraction (SPE) and determined using HPLC method. Chromatographic separation was achieved on a reversed-phase Phenomenex C18 column with the mobile phase of methanol-acetonitrile-formic acid water solution (0.05% formic acid) and gradient elution, temperature of bar was 24 degrees C, flow rate was 0.8 mL x min(-1).
The method showed excellent linearity over the concentration range 0.266-53.1 mg x L(-1) of astilbin (r = 0.996). The extract recoveries were from 79.0% to 89.1%. Significant diffenerce in pharmacokinetic parameter of astilbin including t1/2, Cmax, AUC(0-t), AUC(0-infinity) and MRT were obtained through non-compartment model after oral administration of YXL prescription comparing with Smilacis Glabrae Rhizoma.
The method was applied to a pharmacokinetic study of astilbin. It indicated that the bioavailability of astilbin in YXL enhanced in rats and one of the reasons may be that components of prescription affect the pharmacokinetics of astilbin in vivo.
建立固相萃取-高效液相色谱法测定大鼠血清中落新妇苷含量，并探讨银屑灵（YXL）方及土茯苓对有效成分药代动力学特征的影响。
将雄性Sprague-dawley大鼠分别给予YXL方和土茯苓。于不同时间点，采用固相萃取（SPE）法提取血清中落新妇苷浓度，并用高效液相色谱法测定。色谱分离采用反相Phenomenex C18柱，流动相为甲醇-乙腈-甲酸水溶液（0.05%甲酸），梯度洗脱，柱温24℃，流速0.8 mL·min-1。
落新妇苷在0.26653.1 mg·L-1浓度范围内线性关系良好（r = 0.996）。提取回收率为79.0%89.1%。与土茯苓相比，口服YXL方后，通过非房室模型得到落新妇苷药代动力学参数t1/2、Cmax、AUC(0-t)、AUC(0-∞)和MRT有显著差异。
该方法应用于落新妇苷的药代动力学研究。结果表明，YXL方中落新妇苷在大鼠体内的生物利用度提高了，其原因之一可能是方中成分影响了落新妇苷在体内的药代动力学。
