Kirkeby S
Department of Oral Medicine, Dental School, Panum Institute, University of Copenhagen, Denmark.
Biotech Histochem. 2013 Jan;88(1):19-26. doi: 10.3109/10520295.2012.724084. Epub 2012 Sep 24.
Expression of mucins in cells and tissues is of great diagnostic and prognostic importance, and immunohistochemistry frequently is used to detect them. Reports concerning mucin localization in sections sometimes are conflicting, however, partly because immunogenic regions of the mucin molecule may be masked and thus not available for binding to an antibody. We modified carbohydrates in tissue sections chemically to enhance the binding of monoclonal mucin antibodies and of the lectin, Vicia villosa B4, to human tissue. The immunohistochemical localization of MUC1 and the simple mucin-type antigens, Tn and sialyl-Tn, was influenced by oxidation with periodic acid and by β-elimination before incubation. In some epithelial cells the staining was prevented by these procedures while in other cells it was evident. It appears that chemical modification can either destroy some antigen binding sites or unmask cryptic antigen binding sites in the mucin molecule and thereby make them accessible for immunohistochemical detection.
黏蛋白在细胞和组织中的表达具有重要的诊断和预后意义，免疫组织化学经常用于检测它们。然而，有关黏蛋白在切片中定位的报道有时相互矛盾，部分原因是黏蛋白分子的免疫原性区域可能被掩盖，因而无法与抗体结合。我们对组织切片中的碳水化合物进行化学修饰，以增强单克隆黏蛋白抗体和凝集素（野豌豆B4）与人组织的结合。在孵育前，高碘酸氧化和β-消除对MUC1以及简单黏蛋白型抗原Tn和唾液酸Tn的免疫组织化学定位产生了影响。在一些上皮细胞中，这些操作会阻止染色，而在其他细胞中则染色明显。化学修饰似乎既能破坏黏蛋白分子中的一些抗原结合位点，也能暴露隐蔽的抗原结合位点，从而使其可用于免疫组织化学检测。
