载脂蛋白 A-V 对肝细胞脂滴形成的影响。
Influence of apolipoprotein A-V on hepatocyte lipid droplet formation.
机构信息
Children's Hospital Oakland Research Institute, 5700 Martin Luther King Jr. Way, Oakland, CA 94609, USA.
出版信息
Biochem Biophys Res Commun. 2012 Oct 19;427(2):361-5. doi: 10.1016/j.bbrc.2012.09.065. Epub 2012 Sep 18.
Apolipoprotein A-V (apoA-V) is postulated to modulate intra-hepatic triglyceride (TG) trafficking. Stably transfected McA-RH7777 hepatocarcinoma cells expressing human apoA-V displayed enhanced neutral lipid staining while conditioned media from these cells had 40±8% less TG than cells transfected with a control vector. To obtain homogeneous cell lines expressing different amounts of apoA-V, a strategy of clonal selection was pursued. Immunoblot analysis of two distinct apoA-V stable cell lines yielded one that expresses low amounts of apoA-V and another that expresses higher amounts. Confocal fluorescence microscopy of control cells and cells expressing low levels of apoA-V had similar numbers of lipid droplets while cells expressing higher amounts of apoA-V had twice as many lipid droplets, on average. Thus, apoA-V expression promotes lipid droplet accumulation in these cells.
载脂蛋白 A-V（apoA-V）被认为可以调节肝内甘油三酯（TG）的转运。稳定转染人 apoA-V 的 McA-RH7777 肝癌细胞显示出增强的中性脂质染色，而来自这些细胞的条件培养基中的 TG 比转染对照载体的细胞少 40±8%。为了获得表达不同量 apoA-V 的同质细胞系，采用了克隆选择策略。对两种不同的 apoA-V 稳定细胞系进行免疫印迹分析，得到了一个表达低量 apoA-V 的细胞系和另一个表达高量 apoA-V 的细胞系。用共聚焦荧光显微镜观察，表达低量 apoA-V 的对照细胞和细胞与表达低量 apoA-V 的细胞具有相似数量的脂滴，而表达高量 apoA-V 的细胞的脂滴数量则平均增加了一倍。因此，apoA-V 的表达促进了这些细胞中脂滴的积累。
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