Guangdong Provincial Institute of Traditional Chinese Medicine, Guangzhou, 510095, China.
Chin J Integr Med. 2014 Jan;20(1):31-5. doi: 10.1007/s11655-012-1243-3. Epub 2012 Sep 23.
To investigate the anti-hyperlipidemic effects of apple polyphenols extract (APE) in Triton WR-1339-induced endogenous hyperlipidemic model.
Firstly, APE was isolated and purified from the pomace of Red Fuji Apple and contents of individual polyphenols in APE were determined using high-performance liquid chromatography-mass spectrometry (HPLC-MS). Secondly, forty male National Institude of Health (NIH) mice were randomly divided into 5 groups with 8 animals in each group. The Fenofibrate Capsules (FC) group and APE groups received oral administration of respective drugs for 7 consecutive days. All mice except those in the normal group were intravenously injected through tail vein with Triton WR-1339 on the 6th day. Serum and livers from all the mice were obtained 18 h after the injection. The changes in serum total cholesterol (TC), triglyceride (TG), lipoprotein lipase (LPL) and hepatic triglyceride lipase (HTGL) were measured by respective kits. Finally, expression of hepatic peroxisome proliferator-activated receptor alpha (PPARα) mRNA was measured by real-time reverse transcription-polymerase chain reaction (RT-PCR) method. RESULTS SERUM TC AND TG LEVELS SIGNIFICANTLY INCREASED IN TRITON WR-1339-INDUCED MODEL GROUP COMPARED WITH THE NORMAL GROUP (P<0.01). ORAL ADMINISTRATION OF APE [200 AND 400 MG/(KG DAY)] DOSE-DEPENDENTLY REDUCED THE SERUM LEVEL OF TG IN HYPERLIPIDEMIC MICE (P<0.01). SERUM LPL AND HTGL ACTIVITIES SIGNIFICANTLY DECREASED IN TRITON WR-1339-INDUCED MODEL GROUP COMPARED WITH THE NORMAL GROUP (P<0.05). ORAL ADMINISTRATION OF APE [200 AND 400 MG/(KG DAY)] DOSE-DEPENDENTLY ELEVATED THE SERUM ACTIVITY OF LPL IN HYPERLIPIDEMIC MICE (P<0.05 OR P<0.01). FURTHERMORE, COMPARED WITH THE NORMAL GROUP, HEPATIC MRNA LEVEL OF PPARα IN THE MODEL GROUP SIGNIFICANTLY DECREASED (P<0.01). ORAL ADMINISTRATION OF APE [200 AND 400 MG/(KG DAY)] DOSE-DEPENDENTLY ELEVATED THE EXPRESSION OF PPARα IN HYPERLIPIDEMIC MICE (P<0.05 OR P<0.01):
APE could reduce TG level via up-regulation of LPL activity, which provides new evidence to elucidate the anti-hyperlipidemic effects of APE.
研究苹果多酚提取物（APE）在三酰甘油诱导的内源性高脂血症模型中的降血脂作用。
首先，从红富士苹果渣中分离纯化出 APE，并采用高效液相色谱-质谱联用（HPLC-MS）法测定 APE 中各多酚的含量。其次，将 40 只雄性 NIH 小鼠随机分为 5 组，每组 8 只。连续 7 天，给予芬氟拉明胶囊（FC）组和 APE 组相应药物灌胃。第 6 天，除正常组外，所有小鼠均经尾静脉注射三酰甘油诱导剂 WR-1339。注射后 18 小时，取所有小鼠的血清和肝脏。采用相应试剂盒测定血清总胆固醇（TC）、甘油三酯（TG）、脂蛋白脂肪酶（LPL）和肝甘油三酯脂肪酶（HTGL）的变化。最后，采用实时逆转录-聚合酶链反应（RT-PCR）法测定肝过氧化物酶体增殖物激活受体α（PPARα）mRNA 的表达。
与正常组相比，三酰甘油诱导的模型组血清 TC 和 TG 水平显著升高（P<0.01）。APE[200 和 400 mg/（kg·d）]剂量依赖性降低高脂血症小鼠血清 TG 水平（P<0.01）。与正常组相比，三酰甘油诱导的模型组血清 LPL 和 HTGL 活性显著降低（P<0.05）。APE[200 和 400 mg/（kg·d）]剂量依赖性升高高脂血症小鼠血清 LPL 活性（P<0.05 或 P<0.01）。此外，与正常组相比，模型组肝组织中 PPARαmRNA 水平显著降低（P<0.01）。APE[200 和 400 mg/（kg·d）]剂量依赖性升高高脂血症小鼠肝组织中 PPARα 的表达（P<0.05 或 P<0.01）。
APE 通过上调 LPL 活性降低 TG 水平，为阐明 APE 的降血脂作用提供了新的证据。
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