Sugiyama Hiroshi, Akazome Yoko, Shoji Toshihiko, Yamaguchi Atsuko, Yasue Masaaki, Kanda Tomomasa, Ohtake Yasuyuki
Fundamental Research Laboratory, Asahi Breweries Limited, I-21, Midori 1-chome, Moriya-shi, Ibaraki 302-0106, Japan.
J Agric Food Chem. 2007 May 30;55(11):4604-9. doi: 10.1021/jf070569k. Epub 2007 Apr 26.
Inhibitory effects of apple polyphenol extract (AP) and procyanidin contained in AP on in vitro pancreatic lipase activity and in vivo triglyceride absorption in mice and humans were examined. AP and procyanidin considerably inhibited in vitro pancreatic lipase activity. However, polyphenols, except for procyanidin, in AP (i.e., catechins, chalcones, and phenol carboxylic acids) showed weak inhibitory activities on pancreatic lipase. Procyanidins separated by normal-phase chromatography according to the degree of polymerization were also examined. Inhibitory effects of procyanidins increased according to the degree of polymerization from dimer to pentamer. On the other hand, pentamer or greater procyanidins showed maximal inhibitory effects on pancreatic lipase. These results suggested that with respect to in vitro pancreatic lipase inhibition, the degree of polymerization was an important factor and oligomeric procyanidin mainly contributed. Next, we performed a triglyceride tolerance test in mice and humans. Simultaneous ingestion of AP and triglyceride significantly inhibited an increase of plasma triglyceride levels in both models. These results suggested that the oligomeric procyanidins contained in AP inhibited triglyceride absorption by inhibiting pancreatic lipase activity in mice and humans.
研究了苹果多酚提取物（AP）及其所含原花青素对小鼠和人体体外胰脂肪酶活性及体内甘油三酯吸收的抑制作用。AP和原花青素能显著抑制体外胰脂肪酶活性。然而，AP中的多酚（即儿茶素、查耳酮和酚酸）除原花青素外，对胰脂肪酶的抑制活性较弱。还研究了通过正相色谱法根据聚合度分离的原花青素。原花青素对胰脂肪酶的抑制作用随聚合度从二聚体到五聚体而增加。另一方面，五聚体及以上的原花青素对胰脂肪酶表现出最大抑制作用。这些结果表明，就体外胰脂肪酶抑制而言，聚合度是一个重要因素，低聚原花青素起主要作用。接下来，我们在小鼠和人体中进行了甘油三酯耐量试验。在两个模型中，同时摄入AP和甘油三酯均显著抑制了血浆甘油三酯水平的升高。这些结果表明，AP中所含的低聚原花青素通过抑制小鼠和人体的胰脂肪酶活性来抑制甘油三酯的吸收。
