Komnick H, Achenbach U
Eur J Cell Biol. 1979 Oct;20(1):92-100.
Na+/K+-ATPase localization in the rectal wall of larval Aeshna cyanea (Insecta, Odonata) was studied with histochemical precipitation techniques and 3H-ouabain autoradiography in conjunction with biochemical measurements of enzyme activities and radiospectrometry of 3H-ouabain binding, respectively. The NPP-strontium and ATP-lead methods led to complete inhibition of Na+/K+-ATPase in this organ and hence to unreliable histochemical results. The 3H-ouabain binding technique revealed sodium pump sites at the basolateral plasma membranes of the absorptive rectal chloride epithelia.
运用组织化学沉淀技术和³H-哇巴因放射自显影技术，并分别结合酶活性的生化测定以及³H-哇巴因结合的放射光谱测定，研究了蓝纹豆娘幼虫（昆虫纲，蜻蜓目）直肠壁中钠钾ATP酶的定位。NPP-锶法和ATP-铅法导致该器官中钠钾ATP酶完全被抑制，因此组织化学结果不可靠。³H-哇巴因结合技术揭示了吸收性直肠氯化物上皮细胞基底外侧质膜上的钠泵位点。
