Fargnoli J, Kunisada T, Fornace A J, Schneider E L, Holbrook N J
Laboratory of Molecular Genetics, National Institute on Aging, Baltimore, MD 21224.
Proc Natl Acad Sci U S A. 1990 Jan;87(2):846-50. doi: 10.1073/pnas.87.2.846.
The effect of aging on the induction of heat shock protein 70 (HSP70)-encoding gene expression by elevated temperatures was studied in cultures of lung- or skin-derived fibroblasts from young (5 mo) and old (24 mo) male Wistar rats. Although the kinetics of the heat shock response were found to be similar in the two age groups, we observed lower levels of induction of HSP70 mRNA and HSP70 protein in confluent primary lung and skin fibroblast cultures derived from aged animals. Additional experiments with freshly excised lung tissue showed a similar age-related decline in the heat-induced expression of HSP70.
在来自年轻（5个月）和老年（24个月）雄性Wistar大鼠的肺或皮肤来源的成纤维细胞培养物中，研究了衰老对高温诱导热休克蛋白70（HSP70）编码基因表达的影响。尽管发现两个年龄组的热休克反应动力学相似，但我们观察到，来自老年动物的汇合原代肺和皮肤成纤维细胞培养物中，HSP70 mRNA和HSP70蛋白的诱导水平较低。对新鲜切除的肺组织进行的额外实验表明，热诱导的HSP70表达也有类似的与年龄相关的下降。
