Kline S C, Hotchkiss R N, Randolph M A, Weiland A J
Department of Orthopedic Surgery, Johns Hopkins University School of Medicine.
Plast Reconstr Surg. 1990 Feb;85(2):273-80. doi: 10.1097/00006534-199002000-00017.
The influence of age and maturity on longitudinal skeletal growth was studied using heterotopic limb transplantation with microvascular anastomoses between syngeneic male Lewis rats of different ages. Longitudinal growth of the tibia and growth plate morphology were compared after transplantation between isochronografts (juvenile-to-juvenile and adult-to-adult), heterochronografts (juvenile-to-adult), and unoperated limbs. Four animals comprised each experimental group, except for the juvenile-to-adult heterochronograft group, which had six animals. Limbs were measured at 2-week intervals until sacrifice at 7 weeks. All transplanted limbs demonstrated significant longitudinal growth with maintenance of growth plate morphology and columnar organization. Despite being subjected to an adult hormonal environment, the juvenile tibias transplanted into adult hosts grew 12.0 +/- 1 mm--not significantly different from the unoperated juvenile controls, which grew 12.2 +/- 1 mm. Adult isochronografts showed continued growth of 3.1 mm, which was not significantly different from the unoperated control. Juvenile isochronografts demonstrated decreased growth when compared to unoperated limbs. Maintenance of growth in juvenile limbs transplanted to adults suggests a permissive effect of the hormonal milieu and that ultimate skeletal length is primarily determined by factors inherent in each physis. The use of vascularized transplantation of limbs in syngeneic animals of different ages offers a unique method of altering the hormonal and maturational milieu of the growth plate.
利用不同年龄的同基因雄性Lewis大鼠之间的微血管吻合异位肢体移植，研究年龄和成熟度对骨骼纵向生长的影响。比较了等时移植组（幼年对幼年和成年对成年）、异时移植组（幼年对成年）和未手术肢体移植后胫骨的纵向生长和生长板形态。每个实验组有4只动物，但幼年对成年异时移植组有6只动物。每隔2周测量一次肢体，直至7周时处死。所有移植肢体均表现出显著的纵向生长，生长板形态和柱状结构得以维持。尽管处于成年激素环境中，但移植到成年宿主的幼年胫骨生长了12.0±1毫米，与未手术的幼年对照组（生长了12.2±1毫米）无显著差异。成年等时移植组显示继续生长3.1毫米，与未手术对照组无显著差异。与未手术肢体相比，幼年等时移植组生长减缓。移植到成年动物的幼年肢体生长的维持表明激素环境具有允许作用，并且最终骨骼长度主要由每个生长板固有的因素决定。在不同年龄的同基因动物中使用血管化肢体移植提供了一种改变生长板激素和成熟环境的独特方法。
