General Surgery Center, General Hospital of the Chinese People's Liberation Army, Chengdu Military Region, Chengdu, China.
J Trauma Acute Care Surg. 2012 Sep;73(3):648-53. doi: 10.1097/TA.0b013e318250ad07.
There is not any stable, repeatable, and clinically simulated animal model of isolated pancreatic trauma been established as yet. The aim of our study is to establish a small animal model that mimics the pathophysiology of isolated pancreatic blast trauma and to study the features of traumatic condition in the model.
Rats were divided into three groups: calibrate group, impact group, and control group. The pancreas in the impact group was injured by BIM-III model of the biotical impact machine with the impact pressures of 800 kPa, 600 kPa, 400 kPa, 200 kPa, and 100 kPa. The mortality, intra-abdominal structural changes, amylase (AMS) and lipase (LPS) activities of serum, and pathologic alterations of the pancreas were examined at 24 hours after trauma. We especially observed these changes and the variation of proliferation index in the rats impacted with 400 kPa pressures after 6 hours, 24 hours, 72 hours, and 7 days.
Pancreatic edema, hemorrhage, cell necrosis, and vacuolization were found accompanied with marked increase in serum AMS and LPS. Severity of pancreatic histologic injury was associated with the impact pressure level and varied over time in the 400-kPa group. Applying 400 kPa impact pressure could be able to provide ideal research samples. Pancreatic trauma also induced compensatory proliferation of pancreatic cells.
A practical animal model of isolated pancreatic blunt trauma could be established under 400 kPa pressures. It is ideal for further pathologic and therapeutic study. The combined determination of serum AMS and LPS, particularly the dynamic variation of these enzymes, might assist the diagnosis and prognosis of pancreatic trauma. Further research should focus on the promotion of the cellular regenerative response.
目前尚未建立稳定、可重复且模拟临床情况的胰腺孤立性创伤动物模型。本研究旨在建立模拟胰腺冲击性创伤病理生理学的小动物模型,并研究模型中创伤情况的特征。
将大鼠分为校准组、冲击组和对照组。冲击组大鼠使用生物撞击机的 BIM-III 模型,以 800 kPa、600 kPa、400 kPa、200 kPa 和 100 kPa 的冲击压力损伤胰腺。伤后 24 小时,检查死亡率、腹腔结构变化、血清淀粉酶(AMS)和脂肪酶(LPS)活性以及胰腺病理改变。我们特别观察了在 400 kPa 压力冲击后 6 小时、24 小时、72 小时和 7 天的大鼠这些变化和增殖指数的变化。
发现胰腺水肿、出血、细胞坏死和空泡化,同时血清 AMS 和 LPS 显著增加。胰腺组织学损伤的严重程度与冲击压力水平相关,并在 400-kPa 组随时间变化而变化。施加 400 kPa 的冲击压力可以提供理想的研究样本。胰腺创伤还诱导了胰腺细胞的代偿性增殖。
可以在 400 kPa 压力下建立实用的胰腺钝性创伤动物模型。它非常适合进一步的病理和治疗研究。血清 AMS 和 LPS 的联合测定,特别是这些酶的动态变化,可能有助于胰腺创伤的诊断和预后。进一步的研究应集中在促进细胞再生反应上。