Jones D G, Knox D P
Moredun Research Institute, Edinburgh.
Res Vet Sci. 1990 Jan;48(1):136-7.
The distribution of acetylcholinesterase isoenzymes in the ovine intestinal nematode Trichostrongylus colubriformis was compared with that in chronically infected and worm-free lambs. Total acetylcholinesterase activity in homogenates of adult T colubriformis was resolved into five isoenzyme peaks following gel electrophoresis and specific esterase staining. Two isoenzymes with electrophoretic mobilities similar to those present in adult worm homogenates were detected in mucosal homogenates and plasma extracts from all of six sheep chronically infected with T colubriformis, but not in similar preparations from two uninfected animals.
将绵羊肠道线虫蛇形毛圆线虫中的乙酰胆碱酯酶同工酶分布与慢性感染和未感染蠕虫的羔羊进行了比较。成年蛇形毛圆线虫匀浆中的总乙酰胆碱酯酶活性在凝胶电泳和特异性酯酶染色后被解析为五个同工酶峰。在六只慢性感染蛇形毛圆线虫的绵羊的粘膜匀浆和血浆提取物中检测到两种电泳迁移率与成年蠕虫匀浆中相似的同工酶，但在两只未感染动物的类似制剂中未检测到。
