Li Q G, Wu X R, Li X Z, Yu J, Xia Y, Wang A P, Wang J
Second Department of Respiratory Disease, Jiangxi Provincial People's Hospital, Nanchang, P.R. China.
Genet Mol Res. 2012 Aug 24;11(3):2780-9. doi: 10.4238/2012.August.24.3.
We examined the underlying neural-endocrine mechanisms of asthma associated with respiratory syncytial virus infection. Thirty Sprague-Dawley rats were randomly divided into control group, respiratory syncytial virus (RSV) group, and anti-nerve growth factor (NGF) IgG group. An RSV infection model was established by nasal drip once a week. In the anti-NGF antibody intervention group, each rat was given an intraperitoneal injection of anti-NGF IgG 3 h before RSV infection. Optical microscopy and transmission electron microscopy were used to observe the structural changes in adrenal medulla cells. Changes in adrenaline and norepinephrine in serum were detected by ELISA. NGF expression was assayed by immunohistochemistry. Expression differences in synaptophysin mRNA were detected by RT-PCR. Transmission electron microscopy displayed widened adrenal medulla intercellular spaces, reduced chromaffin particle concentration, and increased mitochondria in the RSV infection group. At the same time, NGF expression was increased in the RSV infection group significantly. In addition, the adrenaline concentration was significantly decreased compared with the control and anti-NGF antibody groups. Synaptophysin mRNA expression was significantly increased in the RSV infection and anti-NGF antibody groups. However, compared with the RSV infection group, synaptophysin mRNA expression was significantly decreased in the anti-NGF antibody group. We conclude that RSV infection could induce adrenal medulla cell differentiation to nerve cells by over-expression of NGF, resulting in the decreased endocrine function found in asthma progression.
我们研究了与呼吸道合胞病毒感染相关的哮喘潜在神经内分泌机制。将30只Sprague-Dawley大鼠随机分为对照组、呼吸道合胞病毒（RSV）组和抗神经生长因子（NGF）IgG组。通过每周滴鼻一次建立RSV感染模型。在抗NGF抗体干预组中，每只大鼠在RSV感染前3小时腹腔注射抗NGF IgG。采用光学显微镜和透射电子显微镜观察肾上腺髓质细胞的结构变化。用ELISA检测血清中肾上腺素和去甲肾上腺素的变化。用免疫组织化学法检测NGF表达。用RT-PCR检测突触素mRNA的表达差异。透射电子显微镜显示RSV感染组肾上腺髓质细胞间隙增宽、嗜铬颗粒浓度降低、线粒体增多。同时，RSV感染组NGF表达显著增加。此外，与对照组和抗NGF抗体组相比，肾上腺素浓度显著降低。RSV感染组和抗NGF抗体组突触素mRNA表达显著增加。然而，与RSV感染组相比，抗NGF抗体组突触素mRNA表达显著降低。我们得出结论，RSV感染可通过NGF的过度表达诱导肾上腺髓质细胞向神经细胞分化，导致哮喘进展过程中内分泌功能下降。
