Trent J M, Stanbridge E J, McBride H L, Meese E U, Casey G, Araujo D E, Witkowski C M, Nagle R B
University of Arizona, Arizona Cancer Center, Tucson 85724.
Science. 1990 Feb 2;247(4942):568-71. doi: 10.1126/science.2300817.
Chromosome banding analysis of human malignant melanoma has documented the nonrandom alteration of chromosome 6. To determine the relevance of chromosome 6 abnormalities in melanoma, a normal chromosome 6 was directly introduced into melanoma cell lines. The resulting (+6) microcell hybrids were significantly altered in their phenotypic properties in culture and lost their ability to form tumors in nude mice. The loss of the chromosome 6 from melanoma microcell hybrids resulted in the reversion to tumorigenicity of these cells in mice. The introduction of the selectable marker (psv2neo) alone into melanoma cell lines had no effect on tumorigenicity. These results support the idea that one or more genes on chromosome 6 may control the malignant expression of human melanoma.
对人类恶性黑色素瘤进行的染色体显带分析已证明6号染色体存在非随机改变。为了确定6号染色体异常在黑色素瘤中的相关性，将一条正常的6号染色体直接导入黑色素瘤细胞系。由此产生的（+6）微细胞杂种在培养中的表型特性发生了显著改变，并失去了在裸鼠体内形成肿瘤的能力。黑色素瘤微细胞杂种中6号染色体的缺失导致这些细胞在小鼠体内恢复致瘤性。单独将选择标记（psv2neo）导入黑色素瘤细胞系对致瘤性没有影响。这些结果支持这样一种观点，即6号染色体上的一个或多个基因可能控制人类黑色素瘤的恶性表达。
