Wadsworth Center, New York State Department of Health, Albany, NY 12208, USA.
Foodborne Pathog Dis. 2012 Nov;9(11):1028-36. doi: 10.1089/fpd.2012.1202. Epub 2012 Sep 25.
Non-O157 Shiga toxin-producing Escherichia coli (STEC) are emerging pathogens with the potential to cause serious illness and impact public health due to diagnostic challenges. Between 2005 and 2010, the Wadsworth Center (WC), the public health laboratory of the New York State (NYS) Department of Health, requested that Shiga toxin enzyme immunoassay (EIA)-positive stool enrichment broths and/or stool specimens be submitted by clinical and commercial reference laboratories testing NYS patient specimens. A total of 798 EIA-positive specimens were received for confirmation and serotyping, and additionally a subset of STEC was assessed for the presence of six virulence genes (stx1, stx2, eaeA, hlyA, nleA, and nleB) by real-time polymerase chain reaction. We confirmed 591 specimens as STEC, 164 (28%) as O157 STEC, and 427 (72%) as non-O157 STEC. Of the non-O157 STEC serogroups identified, over 70% were O103, O26, O111, O45, O121, or O145. During this time period, WC identified and characterized a total of 1282 STEC received as E. coli isolates, stool specimens, or EIA broths. Overall, the STEC testing identified 59% as O157 STEC and 41% as non-O157 STEC; however, out of 600 isolates submitted to the WC as E. coli cultures, 543 (90%) were identified as O157 STEC. This report summarizes a 6-year study utilizing enhanced STEC testing that resulted in increased identification and characterization of non-O157 STEC in NYS. Continued utilization of enhanced STEC testing may lead to effective and timely outbreak response and improve monitoring of trends in STEC disease epidemiology.
非 O157 型志贺毒素产大肠埃希菌（STEC）是新兴的病原体，由于诊断挑战，有可能导致严重疾病并影响公共健康。在 2005 年至 2010 年期间，Wadsworth 中心（WC），即纽约州（NYS）卫生部的公共卫生实验室，要求临床和商业参考实验室对 NYS 患者标本进行志贺毒素酶免疫分析（EIA）阳性粪便增菌培养物和/或粪便标本进行检测，并将 EIA 阳性粪便增菌培养物和/或粪便标本提交给 WC。共收到 798 份 EIA 阳性标本进行确认和血清分型，此外，还评估了一部分 STEC 是否存在 6 种毒力基因（stx1、stx2、eaeA、hlyA、nleA 和 nleB），采用实时聚合酶链反应。我们确认了 591 份标本为 STEC，164 份（28%）为 O157 STEC，427 份（72%）为非 O157 STEC。在鉴定的非 O157 STEC 血清群中，超过 70%为 O103、O26、O111、O45、O121 或 O145。在此期间，WC 共鉴定和表征了作为大肠埃希菌分离物、粪便标本或 EIA 增菌肉汤接收的总计 1282 株 STEC。总体而言，STEC 检测鉴定出 59%为 O157 STEC，41%为非 O157 STEC；然而，600 株作为大肠埃希菌培养物提交给 WC 的分离物中，有 543 株（90%）被鉴定为 O157 STEC。本报告总结了一项为期 6 年的研究，该研究利用增强的 STEC 检测，导致纽约州非 O157 STEC 的鉴定和表征增加。持续利用增强的 STEC 检测可能会导致有效的及时爆发应对，并改善对 STEC 疾病流行病学趋势的监测。
