United States Department of Agriculture, Animal and Plant Health Inspection Service-Veterinary Services, Centers for Epidemiology and Animal Health, Fort Collins, Colorado 80526, USA.
Foodborne Pathog Dis. 2013 Apr;10(4):392-6. doi: 10.1089/fpd.2012.1289. Epub 2013 Mar 4.
While efforts to control foodborne illness associated with the Shiga toxin-producing Escherichia coli (E. coli) O157 through processes and procedures implemented at harvest facilities have been very successful, there is concern about the burden of illness associated with other Shiga toxin-producing E. coli. The U.S. Department of Agriculture Food Safety and Inspection Service announced plans to classify an additional six non-O157 Shiga toxin-producing E. coli as adulterants. Little is known about the prevalence and distribution of these E. coli in the animal production environment. An investigation of the prevalence of O157 and the six major non-O157 E. coli serogroups was conducted in 21 feedlots over the period July 2011 to October 2011. Individual fecal swabs were collected from cattle approximately 60 days after their arrival in the feedlot and were pooled for evaluation using a polymerase chain reaction assay to identify the presence of seven E. coli O-types (O157, O45, O103, O121, O145, O26, and O111) and four virulence genes (stx1, stx2, eaeA, and ehxA). Overall, 1145 fecal pools were evaluated, with 506 (44.2%) being positive for one or more of the E. coli O-serogroups. The pool prevalences for E. coli O157, O45, O26, O103, O121, O145, and O111 were 19.7%, 13.8%, 9.9%, 9.3%, 5.5%, 1.1%, and 0.5%, respectively. Nearly all pools were positive for ehxA (99.7%) or stx2 (98.6%). The pool level prevalence for stx1 and eae was 65.5% and 69.3%, respectively. Pools that were positive for one or more of the other E. coli O-serogroups were 1.37 times more likely to be positive for E. coli O157. Conversely, pools that were positive for E. coli O157 were 1.43 times more likely to be positive for at least one of the other E. coli O-serogroups evaluated. These data will be useful to understand the expected prevalence of potential Shiga toxin-producing E. coli in cattle feedlots.
尽管通过在收获设施实施的过程和程序来控制与产志贺毒素大肠杆菌 (E. coli) O157 相关的食源性疾病已取得非常成功，但人们仍对与其他产志贺毒素大肠杆菌相关的疾病负担感到担忧。美国农业部食品安全检验局宣布计划将另外六种非 O157 产志贺毒素大肠杆菌列为掺杂物。目前对于这些大肠杆菌在动物生产环境中的流行情况和分布情况知之甚少。在 2011 年 7 月至 2011 年 10 月期间，对 21 个饲养场中的 O157 和六种主要非 O157 大肠杆菌血清群的流行情况进行了调查。大约在牛进入饲养场 60 天后，从牛身上采集单独的粪便拭子，并将其汇集在一起，使用聚合酶链反应检测方法进行评估，以确定七种大肠杆菌 O 型（O157、O45、O103、O121、O145、O26 和 O111）和四种毒力基因（stx1、stx2、eaeA 和 ehxA）的存在情况。总体而言，共评估了 1145 个粪便样本池，其中 506 个（44.2%）对一种或多种大肠杆菌 O 血清群呈阳性。大肠杆菌 O157、O45、O26、O103、O121、O145 和 O111 的样本池流行率分别为 19.7%、13.8%、9.9%、9.3%、5.5%、1.1%和 0.5%。几乎所有样本池均为 ehxA（99.7%）或 stx2（98.6%）阳性。stx1 和 eae 的样本池流行率分别为 65.5%和 69.3%。对一种或多种其他大肠杆菌 O 血清群呈阳性的样本池，其对大肠杆菌 O157 呈阳性的可能性增加 1.37 倍。相反，对大肠杆菌 O157 呈阳性的样本池，其对所评估的其他大肠杆菌 O 血清群中的至少一种呈阳性的可能性增加 1.43 倍。这些数据将有助于了解在牛饲养场中预期的产志贺毒素大肠杆菌的流行情况。
