J. Haber Institute of Catalysis and Surface Chemistry Polish Academy of Science, Cracow, Poland.
Colloids Surf B Biointerfaces. 2013 Jan 1;101:442-9. doi: 10.1016/j.colsurfb.2012.07.007. Epub 2012 Jul 20.
Adsorption of human serum albumin (HSA) on mica at pH 3.5 (0.15M NaCl) was studied using in situ streaming potential measurements, AFM imaging and XPS. Results obtained by streaming potential were consistent with AFM measurements and theoretical predictions based on the random sequential adsorption model. These results in combination with complementary data derived from XPS allowed one to determine both the kinetics of adsorption and the maximum coverage of irreversibly bound HSA, which was close to 1.6 mg m(-2) (dimensionless coverage 0.45). It was concluded that HSA adsorption on mica at pH 3.5 occurred under side-on, electrostatically controlled mechanisms with no tendency to multilayer formation. Such irreversible bound HSA monolayers of well-defined coverage can find applications for performing efficient immunological tests, designing biomaterials surfaces and biosensors.
在 pH 3.5（0.15M NaCl）下，使用原位流动电势测量、原子力显微镜成像和 XPS 研究了人血清白蛋白（HSA）在云母上的吸附。流动电势的结果与 AFM 测量和基于随机顺序吸附模型的理论预测一致。这些结果与 XPS 得出的补充数据相结合，使我们能够确定吸附的动力学和不可逆结合的 HSA 的最大覆盖率，接近 1.6mg/m²（无量纲覆盖率 0.45）。得出的结论是，在 pH 3.5 下，HSA 在云母上的吸附是在侧位、静电控制的机制下发生的，没有形成多层的趋势。这种具有明确覆盖率的不可逆结合的 HSA 单层可以应用于进行高效的免疫测试、设计生物材料表面和生物传感器。
