秋水仙碱对前列腺素E1刺激人淋巴母细胞和正常淋巴细胞中环磷酸腺苷1形成的影响。
Effect of colchicine on PGE1 stimulation of cAMP1 formation in human lymphoblastoid and normal lymphocytes.
作者信息
Stolc V
出版信息
Exp Cell Biol. 1979;47(6):401-8.
PGE1 increased cAMP level in human lymphoblastoid cells (RPMI 1788) after 5-60 min of incubation at 37 degrees C. A gradual decrease of cAMP concentration was found at the later time intervals. Colchicine significantly potentiated the stimulatory effect of PGE1, although it did not have any effect on cAMP level in control lymphoblastoid cells. The maximal effect of colchicine on PGE1 stimulation of cAMP formation was at the 0.1-1.0 microM level. Human lymphocytes also responded with increased cAMP formation to colchicine addition. In contrast, no stimulatory effect of colchicine was found in human granulocytes.
在37摄氏度孵育5 - 60分钟后，前列腺素E1（PGE1）可提高人淋巴母细胞（RPMI 1788）中的环磷酸腺苷（cAMP）水平。在随后的时间间隔内发现cAMP浓度逐渐降低。秋水仙碱显著增强了PGE1的刺激作用，尽管它对对照淋巴母细胞中的cAMP水平没有任何影响。秋水仙碱对PGE1刺激cAMP形成的最大作用水平为0.1 - 1.0微摩尔。人淋巴细胞对添加秋水仙碱也有cAMP形成增加的反应。相比之下，在人粒细胞中未发现秋水仙碱的刺激作用。
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