Key Lab. of Advanced Materials Technology of Education Ministry, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, PR China.
Colloids Surf B Biointerfaces. 2013 Feb 1;102:457-65. doi: 10.1016/j.colsurfb.2012.02.018. Epub 2012 Feb 25.
Reducing platelet adhesion, controlling platelet distribution and decreasing degree of platelet activation are very important for improving blood compatibility of cardiovascular implants. In this study, the micropattern of heparin and fibronectin mixture (Hep-Fn) embedded within a cell-resistant α-methoxy-poly(ethylene glycol)-ω-succinimidyl carbonate (mPEG-SC) background was fabricated on titanium (Ti) surface. Firstly, the Ti sample was activated by NaOH solution and treated with ethanol solution of (3-aminopropyl)-triethoxysilane (APTE). Secondly, microtransfer molding (μTM) was used to pattern mPEG-SC for resisting protein absorption and platelet adhesion on silanized Ti surface. Finally, Hep-Fn mixture was absorbed on the areas uncovered by mPEG-SC to form a surface for selective adhesion of platelet. Scanning electron microscopy (SEM) showed the surface morphology of the patterned Ti, Fourier transform infrared spectroscopy (FTIR) demonstrated the existence of Hep-Fn and mPEG-SC on the modified Ti surface. The platelet adhesion test was used to evaluate adhesion, activation and distribution of platelets on the bare Ti samples with or without biomolecule micropattern. The results indicated that the patterned samples could effectively reduce platelet adhesion, control platelet distribution and influence platelet activation compared with the non-patterned pure Ti sample. The micropattern sample, P25/10 with a geometry of 25 μm mPEG-SC (ridges) and 10 μm Hep-Fn (grooves), presented better blood compatibility.
减少血小板黏附、控制血小板分布并降低血小板活化程度对于改善心血管植入物的血液相容性非常重要。本研究在钛（Ti）表面构建了肝素和纤连蛋白混合物（Hep-Fn）的微图案，其嵌入在抗细胞的α-甲氧基-聚（乙二醇）-ω-琥珀酰亚胺碳酸酯（mPEG-SC）背景中。首先，Ti 样品通过 NaOH 溶液进行活化，并使用（3-氨丙基）-三乙氧基硅烷（APTE）的乙醇溶液进行处理。其次，微转移成型（μTM）用于对 mPEG-SC 进行图案化，以抵抗蛋白质吸附和血小板在硅烷化 Ti 表面上的黏附。最后，将 Hep-Fn 混合物吸收到未被 mPEG-SC 覆盖的区域上，以形成血小板选择性黏附的表面。扫描电子显微镜（SEM）显示了图案化 Ti 的表面形态，傅里叶变换红外光谱（FTIR）证明了 Hep-Fn 和 mPEG-SC 存在于改性 Ti 表面上。血小板黏附试验用于评估裸 Ti 样品上有无生物分子微图案时血小板的黏附、活化和分布。结果表明，与非图案化的纯 Ti 样品相比，图案化样品能够有效减少血小板黏附、控制血小板分布并影响血小板活化。具有 25 μm mPEG-SC（脊）和 10 μm Hep-Fn（槽）几何形状的图案化样品 P25/10 表现出更好的血液相容性。
