Institute of Macromolecular Compounds, Russian Academy of Sciences, Bolshoy pr. 31, 199004 St. Petersburg, Russia.
Anal Bioanal Chem. 2013 Mar;405(7):2195-206. doi: 10.1007/s00216-012-6391-y. Epub 2012 Sep 26.
Immobilized enzyme reactors (IMERs) produced by the covalent attachment of ribonuclease A to macroporous methacrylate-based monolithic supports using different experimental approaches are discussed and compared. Enzyme immobilization was carried out by direct covalent binding, as well as through attachment via a polymer spacer. The kinetic properties of an IMER operating in either recirculation mode or zonal elution mode were studied. Additionally, the effect of flow rate on the bioconversion efficiency of each IMER sample was examined.
固定化酶反应器（IMER）通过使用不同实验方法将核糖核酸酶 A 共价连接到大孔甲基丙烯酸酯基整体式载体上制备，本文对其进行了讨论和比较。酶固定化通过直接共价结合以及通过聚合物间隔臂连接进行。研究了在循环模式或区带洗脱模式下操作的 IMER 的动力学特性。此外，还考察了流速对每个 IMER 样品的生物转化效率的影响。
