Schumacher V L, Martel A, Pasmans F, Van Immerseel F, Posthaus H
Institute of Animal Pathology, Vetsuisse Faculty, University of Berne, Längassstrasse 122, 3012 Bern, Switzerland.
Vet Pathol. 2013 Jul;50(4):626-9. doi: 10.1177/0300985812461362. Epub 2012 Sep 24.
Beta toxin (CPB) is known to be an essential virulence factor in the development of lesions of Clostridium perfringens type C enteritis in different animal species. Its target cells and exact mechanism of toxicity have not yet been clearly defined. Here, we evaluate the suitability of a neonatal piglet jejunal loop model to investigate early lesions of C. perfringens type C enteritis. Immunohistochemically, CPB was detected at microvascular endothelial cells in intestinal villi during early and advanced stages of lesions induced by C. perfringens type C. This was first associated with capillary dilatation and subsequently with widespread hemorrhage in affected intestinal segments. CPB was, however, not demonstrated on intestinal epithelial cells. This indicates a tropism of CPB toward endothelial cells and suggests that CPB-induced endothelial damage plays an important role in the early stages of C. perfringens type C enteritis in pigs.
已知β毒素（CPB）是不同动物物种中C型产气荚膜梭菌肠炎病变发展的一种必需毒力因子。其靶细胞和确切毒性机制尚未明确界定。在此，我们评估新生仔猪空肠袢模型对研究C型产气荚膜梭菌肠炎早期病变的适用性。免疫组织化学检测发现，在C型产气荚膜梭菌诱导的病变早期和晚期，肠绒毛微血管内皮细胞中可检测到CPB。这首先与毛细血管扩张有关，随后与受影响肠段的广泛出血有关。然而，在肠上皮细胞上未检测到CPB。这表明CPB对内皮细胞具有嗜性，并提示CPB诱导的内皮损伤在猪C型产气荚膜梭菌肠炎早期起重要作用。
