Zhu Haitao, Yu Liang, Wang Bo
Department of Hepatobiliary, First Affiliated Hospital, Medical School of Xi'an Jiaotong University, Xi'an Shaanxi 710061, China.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2012 Aug;26(8):1012-6.
To review the common methods of isolation and purification of porcine islets and research progress.
Domestic and abroad literature concerning the isolation and purification of porcine islets was reviewed and analyzed thoroughly.
The efficacy of the isolation and purification depends on the selection of donor, the procurement and cryopreservation of high-quality donor pancreas, and the selection and improvement of the operation.
The shortage of transplanted islets could be resolved by the establishment of standardized and optimal process, which may also promote the development of porcine islet xenograft.
综述猪胰岛分离纯化的常用方法及研究进展。
对国内外有关猪胰岛分离纯化的文献进行全面回顾与分析。
分离纯化的效果取决于供体的选择、高质量供体胰腺的获取与冷冻保存以及手术操作的选择与改进。
建立标准化、优化的流程可解决移植胰岛短缺问题，也可能推动猪胰岛异种移植的发展。
