Zhang Ying, Zhang Jin, Bo Shu-Ying, Wang Guo-Zhi, Xin Xiao-Fang
National Institute for Food and Drug Control, Beijing 100050, China.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2012 Jun;24(3):284-9.
To investigate the dynamic changes of SEA-induced specific IgG, IgM in sera of BALB/c mice infected with Schistosoma japonicum in 18 weeks.
After mice were infected with S. japonicum cercarial for 2 weeks, the sera were collected from 2 to 18 weeks post-infection. The serum levels of SEA-specific IgG and IgM antibodies were measured respectively by ELISA, and the different fractions of IgG and IgM antibodies were identified by the Western blotting method.
The ELISA results showed that the serum levels of SEA-specific IgG increased 5, 6, 9, 11 week, after the infection, and SEA-specific IgM increased obviously 5, 9 weeks after the infection. The Western blotting results showed that 140, 180 kDa molecules were recognized by IgG antibodies in the mouse sera 4 weeks after the infection. The specific IgG antibodies of 43, 50 kDa antigens appeared 5 weeks after the infection. 60-130 kDa fractions were recognized by IgG in the sera 6 weeks post-infection, and 38, 73 kDa proteins were recognized by IgG in the sera 9 weeks post-infection. The IgG antibodies of 26, 32, 35, 80 kDa molecules appeared 11 weeks post-infection and reacted strongly 12 weeks post-infection. The IgM antibodies of 100, 140, 180 kDa molecules appeared 3 weeks after the infection, and 73 kDa protein was recognized by sera 6 weeks after the infection, but the reaction became strong 9 weeks after the infection. The 38, 43, 50 kDa proteins induced IgM antibodies in 9-week-infection sera and the reaction became stronger 9 weeks after the infection.
There is a dynamic change in the levels of specific IgG and IgM antibodies induced by S. japonica SEA and the appearance of the antibodies is related to different infection stages. The 43, 50, 10, 140, 180 kDa antigens might have the potential value of early immunodiagnosis. The 73 kDa antigen shows high diagnostic value in both acute and chronic schistosomiasis. The 28, 32, 35, 38, 80 kDa antigens are not only the diagnostic molecules for chronic schistosomiasis, but they may also have therapeutic effects, and in addition, they may be the candidate vaccines of the disease.
研究日本血吸虫感染18周内BALB/c小鼠血清中SEA诱导的特异性IgG、IgM的动态变化。
小鼠感染日本血吸虫尾蚴2周后，于感染后2至18周采集血清。分别采用ELISA法检测血清中SEA特异性IgG和IgM抗体水平，并用Western印迹法鉴定IgG和IgM抗体的不同组分。
ELISA结果显示，感染后第5、6、9、11周血清中SEA特异性IgG水平升高，感染后第5、9周SEA特异性IgM明显升高。Western印迹结果显示，感染后4周小鼠血清中的IgG抗体可识别140、180 kDa分子。感染后5周出现43、50 kDa抗原的特异性IgG抗体。感染后6周血清中的IgG可识别60 - 130 kDa组分，感染后9周血清中的IgG可识别38、73 kDa蛋白。感染后11周出现26、32、35、80 kDa分子的IgG抗体，感染后12周反应强烈。感染后3周出现100、140、180 kDa分子的IgM抗体，感染后6周血清可识别73 kDa蛋白，但感染后9周反应增强。感染9周血清中38、43、50 kDa蛋白诱导产生IgM抗体，感染后9周反应更强。
日本血吸虫SEA诱导的特异性IgG和IgM抗体水平存在动态变化，抗体的出现与不同感染阶段有关。43、50、10、140、180 kDa抗原可能具有早期免疫诊断的潜在价值。73 kDa抗原在急性和慢性血吸虫病中均显示出较高的诊断价值。28、32、35、38、80 kDa抗原不仅是慢性血吸虫病的诊断分子，还可能具有治疗作用，此外，它们可能是该疾病的候选疫苗。
