Brainard Benjamin M, Koenig Amie, Babski Danielle M, Blong April E, Scherk Jordan R
Department of Small Animal Medicine and Surgery, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA.
Am J Vet Res. 2012 Oct;73(10):1577-82. doi: 10.2460/ajvr.73.10.1577.
To evaluate the pharmacodynamic effects of dalteparin in dogs by means of viscoelastic coagulation monitoring with a thromboelastograph and a dynamic viscoelastic coagulometer.
6 healthy adult mixed-breed dogs.
Dalteparin (175 U/kg, SC, q 12 h) was administered for 4 days (days 1 through 4). Viscoelastic coagulation monitoring was performed hourly on the first and last days of treatment and included intermittent measurement of anti-activated coagulation factor X activity (AXA).
Dalteparin administration resulted in progressive hypocoagulability. On both day 1 and 4, activated clotting time and clot rate for the dynamic viscoelastic coagulometer differed significantly from baseline values, whereas the platelet function parameter did not change on day 1 but did on day 4. The R (reaction time), time from reaction time until the amplitude of the thromboelastography tracing is 20 mm, α-angle, and maximum amplitude differed from baseline values on days 1 and 4, although many thromboelastographic variables were not determined. The AXA was increased from baseline values at 3 and 6 hours after administration of the dalteparin injection on days 1 and 4, and all dogs had AXA values between 0.5 and 1.0 U/mL at 2 and 4 hours after administration. The AXA correlated well with activated clotting time (r = 0.761) and with R (r = 0.810), when values were available. Thromboelastography could not be used to distinguish AXA > 0.7 U/mL.
Viscoelastic coagulation monitoring with strong coagulation activators may be used to monitor treatment with dalteparin in healthy dogs.
通过使用血栓弹力图仪和动态粘弹性凝血仪进行粘弹性凝血监测，评估达肝素对犬的药效学作用。
6只健康成年杂种犬。
给予达肝素（175 U/kg，皮下注射，每12小时1次），持续4天（第1天至第4天）。在治疗的第1天和最后1天每小时进行粘弹性凝血监测，包括间歇性测量抗活化凝血因子X活性（AXA）。
给予达肝素导致凝血功能逐渐降低。在第1天和第4天，动态粘弹性凝血仪的活化凝血时间和凝血速率与基线值相比均有显著差异，而血小板功能参数在第1天未发生变化，但在第4天发生了变化。尽管许多血栓弹力图变量未测定，但在第1天和第4天，反应时间（R）、从反应时间到血栓弹力图描记幅度达到20 mm的时间、α角和最大幅度与基线值不同。在第1天和第4天，给予达肝素注射后3小时和6小时，AXA较基线值升高，所有犬在给药后2小时和4小时的AXA值在0.5至1.0 U/mL之间。当有可用数据时，AXA与活化凝血时间（r = 0.761）和R（r = 0.810）相关性良好。血栓弹力图不能用于区分AXA>0.7 U/mL。
使用强凝血激活剂进行粘弹性凝血监测可用于监测健康犬接受达肝素的治疗情况。
