Higashimoto Y, Himeno S, Shinomura Y, Kuroshima T, Nagao K, Tarui S
Second Department of Internal Medicine, Osaka University Medical School, Japan.
Am J Gastroenterol. 1990 Feb;85(2):132-7.
Urinary and plasma gastrin immunoreactivities in normal subjects were studied by radioimmunoassays using three region-specific antisera. Urinary excretion of NH2-terminal big gastrin immunoreactivity (NT G-34-IR) in the fasting state (0.79 +/- 0.17 pmol/kg/h, mean +/- SE) was several hundred times as much as that of either COOH-terminal gastrin or gastrin/cholecystokinin immunoreactivity. Urinary NT G-34-IR increased significantly after feeding, and correlated closely with integrated plasma NT G-34-IR. Renal clearance of NT G-34-IR was 62.4 +/- 7.9 ml/min and about one hundred times greater than that of any other gastrin immunoreactivities, indicating that there exist different catabolic pathways of gastrin peptides in the kidney. Gel filtration of urine extract revealed that a single giant peak of NT G-34-IR eluted in a later position than NH2-terminal heptadecapeptide of big gastrin. These results suggest that most of plasma NT G-34-IR is excreted in urine, and urinary excretion of NT G-34-IR reflects well-integrated plasma NT G-34-IR. Therefore, urinary NT G-34-IR may serve as a feasible indicator for gastrin secretion.
使用三种区域特异性抗血清，通过放射免疫分析法研究了正常受试者尿液和血浆中的胃泌素免疫反应性。空腹状态下，尿液中氨基末端大胃泌素免疫反应性（NT G-34-IR）的排泄量（0.79±0.17 pmol/kg/h，平均值±标准误）是羧基末端胃泌素或胃泌素/胆囊收缩素免疫反应性排泄量的数百倍。进食后，尿液中NT G-34-IR显著增加，且与血浆中NT G-34-IR的积分密切相关。NT G-34-IR的肾脏清除率为62.4±7.9 ml/min，约为其他任何胃泌素免疫反应性清除率的100倍，这表明肾脏中存在胃泌素肽的不同分解代谢途径。尿液提取物的凝胶过滤显示，NT G-34-IR的单一巨大峰洗脱位置比大胃泌素的氨基末端十七肽更靠后。这些结果表明，血浆中大部分NT G-34-IR通过尿液排泄，尿液中NT G-34-IR的排泄很好地反映了血浆中NT G-34-IR的整体情况。因此，尿液中NT G-34-IR可能是胃泌素分泌的一个可行指标。
