乌克兰乳腺癌患者和健康个体的染色体放射敏感性
Chromosomal radiosensitivity in Ukrainian breast cancer patients and healthy individuals.
作者信息
Ryabchenko N M, Glavin O A, Shtefura V V, Anikushko M F
机构信息
RE Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine.
出版信息
Exp Oncol. 2012 Jul;34(2):121-4.
AIM
Recent studies showed that increased chromosomal damage induced by ionizing radiation is observed among patients with different tumor types. The aim of the study was evaluation of chromosomal radiosensitivity in breast cancer (BC) patients (n = 37) and healthy women (n = 44).
METHODS
Chromosomal radiosensitivity was assessed with G0 and G2 assay. For G0 assay lymphocytes were exposed in vitro to 1,5 Gy of X-rays before culture setting. For G2 assay lymphocytes were irradiated with 0,5 Gy of X-rays after 47 h of incubation.
RESULTS
Significant differences in mean scores both of G0 and G2 assay between breast cancer patients and controls were observed indicating the increased chromosomal radiosensitivity of lymphocytes of cancer patients. 11% of healthy women and 38% of BC patients were determined to be radiosensitive with G2 assay.
CONCLUSION
Obtained results support the concept of association between elevated individual G2 chromosomal radiosensitivity and predisposition to BC.
目的
近期研究表明，在不同肿瘤类型的患者中观察到电离辐射诱导的染色体损伤增加。本研究的目的是评估乳腺癌（BC）患者（n = 37）和健康女性（n = 44）的染色体放射敏感性。
方法
采用G0和G2试验评估染色体放射敏感性。对于G0试验，淋巴细胞在体外培养前暴露于1.5 Gy的X射线。对于G2试验，淋巴细胞在孵育47小时后用0.5 Gy的X射线照射。
结果
观察到乳腺癌患者和对照组在G0和G2试验的平均得分上存在显著差异，表明癌症患者淋巴细胞的染色体放射敏感性增加。通过G2试验确定，11%的健康女性和38%的BC患者对辐射敏感。
结论
获得的结果支持个体G2染色体放射敏感性升高与患BC易感性之间存在关联的概念。
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