Yaroslavsky A N, Yaroslavsky I V, Goldbach T, Schwarzmaier H J
J Biomed Opt. 1999 Jan;4(1):47-53. doi: 10.1117/1.429920.
We investigated the impact of the scattering phase function approximation on the optical properties of whole human blood determined from integrating sphere measurements using an inverse Monte Carlo technique. The diffuse reflectance Rd and the total transmittance Tt(λ=633 nm) of whole blood samples (Hct=38%) were measured with double-integrating sphere equipment. The scattering phase functions of highly diluted blood samples (Hct=0.1%) were measured using a goniophotometer. We approximated the experimentally determined scattering phase functions with either Henyey-Greenstein (HGPF), Gegenbauer kernel (GKPF), or Mie (MPF) phase functions to preset the anisotropy factor μ¯ for the inverse problem. We have employed HGPF, GKPF, and MPF approximations in the inverse Monte Carlo procedure to derive the absorption coefficient μa and the scattering coefficient μs. To evaluate the obtained data, we calculated the angular distributions of scattered light for optically thick samples and compared the results with goniophotometric measurements. The data presented in this study demonstrate that the employed approximation of the scattering phase function can have a substantial impact on the derived values of μs and μ¯, while μa and the reduced scattering coefficient μs' are much less sensitive to the exact form of the scattering phase function. © 1999 Society of Photo-Optical Instrumentation Engineers.
我们研究了散射相函数近似对使用逆蒙特卡罗技术通过积分球测量确定的全血光学特性的影响。使用双积分球设备测量了全血样本（血细胞比容Hct = 38%）的漫反射率Rd和总透射率Tt(λ = 633 nm)。使用测角光度计测量了高度稀释血样（血细胞比容Hct = 0.1%）的散射相函数。我们用亨耶 - 格林斯坦（HGPF）、盖根堡核（GKPF）或米氏（MPF）相函数对实验确定的散射相函数进行近似，以预设反问题的各向异性因子μ¯。我们在逆蒙特卡罗过程中采用HGPF、GKPF和MPF近似来推导吸收系数μa和散射系数μs。为了评估所得数据，我们计算了光学厚样本的散射光角分布，并将结果与测角光度计测量结果进行比较。本研究中呈现的数据表明，所采用的散射相函数近似对推导得到的μs和μ¯值可能有重大影响，而μa和约化散射系数μs'对散射相函数的精确形式则不太敏感。© 1999 美国光电仪器工程师协会。
