辣根过氧化物酶对刚果红的酶促降解。

Enzymatic degradation of Congo Red by turnip (Brassica rapa) peroxidase.

机构信息

Laboratoire de Biomatériaux et Phénomènes de Transfert, Faculté des Sciences et de la Technologie, Université Yahia Fares de Médéa, Pole Universitaire, RN1, Médéa, 26000, Algeria.

出版信息

Z Naturforsch C J Biosci. 2012 Jul-Aug;67(7-8):429-36. doi: 10.1515/znc-2012-7-811.

DOI:10.1515/znc-2012-7-811

PMID:23016283

Abstract

The enzyme peroxidase is known for its capacity to remove phenolic compounds and aromatic amines from aqueous solutions and also to decolourize textile effluents. This study aims at evaluating the potential of a turnip (Brassica rapa) peroxidase (TP) preparation in the discolouration of textile azo dyes and effluents. An azo dye, Congo Red (CR), was used as a model pollutant for treatment by the enzyme. The effects of various operating conditions like pH value, temperature, initial dye and hydrogen peroxide concentrations, contact time, and enzyme concentration were evaluated. The optimal conditions for maximal colour removal were at pH 2.0, 40 degrees C, 50 mM hydrogen peroxide, 50 mg/l CR dye, and TP activity of 0.45 U/ml within 10 min of incubation time. Analysis of the by-products from the enzymatic treatment by UV-Vis and IR spectroscopy showed no residual compounds in the aqueous phase and a precipitate of polymeric nature.

摘要

过氧化物酶以其从水溶液中去除酚类化合物和芳香胺的能力以及对纺织废水进行脱色的能力而闻名。本研究旨在评估萝卜（Brassica rapa）过氧化物酶（TP）制剂在纺织偶氮染料和废水脱色方面的潜力。使用一种偶氮染料刚果红（CR）作为用酶处理的模型污染物。评估了各种操作条件（如 pH 值、温度、初始染料和过氧化氢浓度、接触时间和酶浓度）的影响。最大颜色去除的最佳条件为 pH 值 2.0、40°C、50mM 过氧化氢、50mg/l CR 染料和 0.45U/ml 的 TP 活性，孵育 10 分钟。通过 UV-Vis 和 IR 光谱对酶处理的副产物进行分析表明，水相中没有残留化合物，并且形成了聚合性质的沉淀物。