Huang Juan, Liu Yan, Ding Tao, Zhang Xiaoyan, Chen Huilan, Shen Chongyu, Wu Bin, Niu Wen
Animal, Plant and Food Inspection Center of Jiangsu Entry-Exit Inspection and Quarantine Bureau, Nanjing 210001, China.
Se Pu. 2012 Jun;30(6):578-83. doi: 10.3724/sp.j.1123.2012.02002.
The quantification method for the determination of kojic acid in foods using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was developed. For solid samples, the kojic acid was extracted with acetonitrile; for liquid samples, they were diluted with water, then deproteinized by the deposition with zinc acetate and potassium ferrocyanide. The analytes were determined by HPLC-MS/MS on a C18 column with 5 mmol/L ammonium acetate/formic acid solution as mobile phases. The analysis of kojic acid was performed under selected reaction monitoring (SRM) mode by selecting one parent ion and two daughter ions as qualitative ions with [13C6]-kojic acid as the internal standard, and the most abundant daughter ion as quantitative ion. The limits of quantification (S/N > 10) were 0.1 mg/kg for the solid samples, and 2.5 mg/kg for the liquid samples. The good linearity (r > 0.99) was achieved for the target compound over the range of 0.1 - 2.0 mg/L. The recoveries at three levels for kojic acid were from 72.6% to 114% with the relative standard deviations no more than 11.4%. The method is simple and practical, and can be applied to most of matrices which may contain kojic acid as food additives. It can meet the qualitative and quantitative requirements for import and export foods.
建立了高效液相色谱-串联质谱法（HPLC-MS/MS）测定食品中曲酸的定量方法。对于固体样品，用乙腈提取曲酸；对于液体样品，用水稀释后，用醋酸锌和亚铁氰化钾沉淀进行脱蛋白处理。采用HPLC-MS/MS在C18柱上进行分析，以5 mmol/L醋酸铵/甲酸溶液为流动相。以[13C6]-曲酸为内标，在选择反应监测（SRM）模式下进行曲酸分析，选择一个母离子和两个子离子作为定性离子，以丰度最高的子离子作为定量离子。固体样品的定量限（S/N>10）为0.1 mg/kg，液体样品为2.5 mg/kg。目标化合物在0.1 - 2.0 mg/L范围内具有良好的线性（r>0.99）。曲酸三个添加水平的回收率为72.6%至114%，相对标准偏差不超过11.4%。该方法简单实用，可应用于大多数可能含有曲酸作为食品添加剂的基质。它能够满足进出口食品的定性和定量要求。
