Center for Stem Cell and Tissue Regeneration, Graduate Institute of Medicine, College of Medicine, San Ming District, 807Kaohsiung, Taiwan.
Curr Mol Med. 2012 Dec;12(10):1340-9. doi: 10.2174/156652412803833625.
Human amnion-derived cells possess great potential for the repair of human neural disorders, and recent studies have broadened the spectrum for applications because they exhibit the characteristics of multipotent stem cells. These cells express embryonic stem cell markers such as Oct4, Nanog, Sox2, SSEA-3, SSEA-4 and Rex1, and can differentiate into multiple primary germ layers both in vitro and in vivo. Moreover, induced pluripotent stem cells have been generated from amnion-derived cells by virus-mediated delivery of three or four pluripotency-relating transcription factors or by the introduction of only one transcription factor with electroporation. Because human amnion-derived cells are readily available, less likely to contain genetic aberrations and can be reprogrammed earlier and more efficiently than differentiated cells, they can be ideal resources as the donor pluripotent stem cells for therapeutic purposes. We discuss here the highlights of recent studies and potential applications of human amnion-derived multipotent stem cells to stem cell biology as well as to regenerative medicine in the field of aging, heart disease, diabetes and neural disorders.
人羊膜来源细胞在人类神经疾病的修复中具有巨大的潜力，最近的研究拓宽了其应用范围，因为它们表现出多能干细胞的特征。这些细胞表达胚胎干细胞标志物，如 Oct4、Nanog、Sox2、SSEA-3、SSEA-4 和 Rex1，并且可以在体外和体内分化为多种原始生殖层。此外，已经通过病毒介导的三种或四种多能性相关转录因子的传递或通过电穿孔引入仅一种转录因子，从羊膜来源细胞中产生诱导多能干细胞。由于人羊膜来源细胞易于获得、不太可能包含遗传异常，并且可以比分化细胞更早且更有效地被重编程，因此它们可以作为治疗目的的供体多能干细胞成为理想的资源。我们在这里讨论了最近的研究的重点以及人羊膜来源的多能干细胞在干细胞生物学以及衰老、心脏病、糖尿病和神经疾病领域的再生医学中的潜在应用。
