Saksirisampant Wilai, Boontanom Parima, Mungthin Mathirut, Tan-Ariya Peerapan, Lamchuan Daoruang, Siripattanapipong Suradej, Leelayoova Saovanee
Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
Trop Biomed. 2012 Sep;29(3):331-8.
A cross-sectional study was performed to determine the prevalence of giardiasis in hilltribe children of 2 different remote districts (Mae-chaem and Hod), Chiang Mai, Northern Thailand from November 2006-April 2007. The overall prevalence of giardiasis was 5.2%. Genetic characterization of Giardia duodenalis isolated from these children was performed using PCR methods specific for small subunit ribosomal rRNA (SSU-rRNA) and glutamate dehydrogenase (gdh) gene. This study shows that the distribution of Giardia assemblages varied in these 2 populations. Assemblage BIV was found predominantly in children from Hod District while assemblage AII was more common in children from Mae-Chaem District. Our result showed that assemblage A was significantly associated with loose/watery stool (p = 0.001). In addition, children harbouring assemblage B had shed a significantly higher number of cysts (p = 0.019) in stools than those infected with assemblage A. Further study on the epidemiology of giardiasis especially risk factors associated with genotyping would help to understand the nature of this disease in each population.
2006年11月至2007年4月，在泰国北部清迈两个不同偏远地区（湄占和霍德）的山地部落儿童中开展了一项横断面研究，以确定贾第虫病的患病率。贾第虫病的总体患病率为5.2%。使用针对小亚基核糖体RNA（SSU-rRNA）和谷氨酸脱氢酶（gdh）基因的PCR方法，对从这些儿童中分离出的十二指肠贾第虫进行基因特征分析。本研究表明，这两个群体中贾第虫组合的分布有所不同。BIV组合主要在霍德地区的儿童中发现，而AII组合在湄占地区的儿童中更为常见。我们的结果表明，A组合与稀便/水样便显著相关（p = 0.001）。此外，携带B组合的儿童粪便中排出的包囊数量明显高于感染A组合的儿童（p = 0.019）。对贾第虫病流行病学，尤其是与基因分型相关的危险因素进行进一步研究，将有助于了解每个群体中这种疾病的本质。
