Zhang Fu-Xiang, Yang Yi-Bin, Ke Gui-Bao, Chen Ying, Xu Xin-Mei, Tan Zhou-Ke, Rong Song
Department of Nephrology, Laboratory of Organ Transplantation, Zunyi Medical College, China.
Dongwuxue Yanjiu. 2012 Oct;33(5):493-7. doi: 10.3724/SP.J.1141.2012.05493.
The purpose of this study was to establish a parabiotic mice model and assess the physiological changes of the mice under the parabiotic state. Thirteen pairs of isogenic partners were studied. The model was created by preparing a bridge of skin and subcutaneous tissues between the two mice starting distal of the elbow joint along the humerus along the lateral costal region until the end of the waist line. Physiological, social and affective qualities of life were studied in the mice through behavioural observations for 120 days following the parabiotic surgery. During the first 2-3 days following the operation, the animals suffered from severe pain and distress. During the following days and weeks, the physiological system began to recover and the animals displayed behavioral adaptations to the parabiotic condition. All animals survived at day 120. At three days post operation, the body weight began to decrease. Following this, the animals experienced a continual body weight recovery and reached pre-surgical measures at about 30 days post op. Forty-eight h post op., faecal corticosterone-metabolites were extremely elevated, but their levels returned to two to four times of levels in control females within 72 hours post op. The faecal corticosterone-metabolite levels decreased near to control values on day 75. Out of the 13 pairs, the blood exchange rate of three parabiotic partners was tested, with the result being normal post op. After 12 weeks, the total blood exchange between both partners needed 63 or 46 or 107 min, respectively. These results demonstrated that the animals could adapt behaviourally to the parabiotic situation. Therefore, this parabiosis mouse model may provide useful insights in many research areas, such as transplantation immunity, hematological system and metabolism, etc.
本研究的目的是建立联体小鼠模型，并评估处于联体状态下小鼠的生理变化。研究了13对同基因配对小鼠。通过在两只小鼠之间从肘关节远端沿肱骨、沿侧肋区域直至腰线末端制备皮肤和皮下组织桥来创建该模型。在联体手术后通过行为观察对小鼠的生理、社交和情感生活质量进行了120天的研究。在手术后的最初2 - 3天，动物遭受严重疼痛和痛苦。在接下来的几天和几周内，生理系统开始恢复，动物表现出对联体状态的行为适应。所有动物在第120天存活。术后三天，体重开始下降。在此之后，动物体重持续恢复，并在术后约30天达到术前水平。术后48小时，粪便皮质酮代谢物极度升高，但在术后72小时内其水平恢复到对照雌性小鼠的两到四倍。粪便皮质酮代谢物水平在第75天降至接近对照值。在13对小鼠中，对3对联体配对小鼠的血液交换率进行了检测，结果术后正常。12周后，两对配对小鼠之间的总血液交换分别需要63分钟、46分钟或107分钟。这些结果表明动物能够在行为上适应联体状态。因此，这种联体小鼠模型可能在许多研究领域提供有用的见解，如移植免疫、血液系统和新陈代谢等。
