Department of Applied Science, University of Arkansas at Little Rock, Little Rock, AR 72204, USA.
Small. 2013 Jan 14;9(1):115-23. doi: 10.1002/smll.201201225. Epub 2012 Sep 28.
Multi-walled carbon nanotubes (CNTs) can affect plant phenotype and the composition of soil microbiota. Tomato plants grown in soil supplemented with CNTs produce two times more flowers and fruit compared to plants grown in control soil. The effect of carbon nanotubes on microbial community of CNT-treated soil is determined by denaturing gradient gel electrophoresis and pyrosequencing analysis. Phylogenetic analysis indicates that Proteobacteria and Bacteroidetes are the most dominant groups in the microbial community of soil. The relative abundances of Bacteroidetes and Firmicutes are found to increase, whereas Proteobacteria and Verrucomicorbia decrease with increasing concentration of CNTs. The results of comparing diversity indices and species level phylotypes (OTUs) between samples showed that there is not a significant affect on bacterial diversity.
多壁碳纳米管（CNTs）会影响植物表型和土壤微生物群落组成。与在对照土壤中生长的植物相比，在添加 CNT 的土壤中生长的番茄植物产生的花和果实数量增加了两倍。通过变性梯度凝胶电泳和焦磷酸测序分析来确定 CNT 处理土壤中微生物群落对碳纳米管的影响。系统发育分析表明，在土壤微生物群落中，变形菌门和拟杆菌门是最主要的群体。随着 CNT 浓度的增加，发现拟杆菌门和厚壁菌门的相对丰度增加，而变形菌门和疣微菌门减少。对样品间多样性指数和种水平分类群（OTUs）的比较结果表明，细菌多样性没有受到显著影响。
