Chen Yan, Zhou Lei, Xin Ran, Wang Jinyan, Jia Xiaobin
Key Laboratory of Chinese Medicine New Drug Delivery Systems, Jiangsu Institute of Traditional Chinese Medicine, Key Laboratory of Chinese Medicine Oral Formulations Drug Delivery Systems, State Administration of Traditional Chinese Medicine, Nanjing 210028, China.
Zhongguo Zhong Yao Za Zhi. 2012 Jul;37(13):2012-6.
To compare the difference of the absorption difference between paeoniflorin monomer and Paeonia lactiflora extracts in rat intestinal canals by multi-channels.
The rat intestinal perfusion model was established. The intestinal perfusate, bile and plasma samples were collected, in combination of the intestinal enzymes incubation test and the partition coefficient determination, to conduct a multi-channel analysis and comparison on absorption difference between paeoniflorin and P. lactiflora extracts.
In the same concentration, permeability coefficient P(eff)* of paeoniflorin in the different intestinal segments of P. lactiflora extract higher than the monomer of paeoniflorin, especially in the jejunum and ileum intestinal segments (P < 0. 05). However, both paeoniflorin monomer and P. lactiflora extracts showed less P(eff)* in four intestinal segments, with the former ranging between 0. 209-0.290 and the latter 0.252-0.333. No paeoniflorin and its metabolin was determined in bile samples, plasma samples and intestinal enzymes incubation samples of paeoniflorin monomer and P. lactiflora extracts.
Compared with the paeoniflorin monomer, P. lactiflora extract showed significantly increase in P(eff)*, which indicated that other ingredients in the extract can improve the absorption of paeoniflorin. However, due to the poor absorption of paeoniflorin, this effect fails to increase the concentration of paeoniflorin in bile and plasma within short period of time.
多通道比较芍药苷单体与白芍提取物在大鼠肠道内吸收差异。
建立大鼠肠灌注模型，收集肠灌注液、胆汁及血浆样品，结合肠酶孵育试验和分配系数测定，对芍药苷与白芍提取物的吸收差异进行多通道分析比较。
相同浓度下，白芍提取物中芍药苷在不同肠段的通透系数P(eff)*高于芍药苷单体，尤其在空肠和回肠段（P<0.05）。但芍药苷单体与白芍提取物在四段肠中P(eff)*均较小，前者为0.209 - 0.290，后者为0.252 - 0.333。芍药苷单体与白芍提取物的胆汁样品、血浆样品及肠酶孵育样品中均未检测到芍药苷及其代谢产物。
与芍药苷单体相比，白芍提取物的P(eff)*显著升高，表明提取物中其他成分可促进芍药苷吸收。但因芍药苷吸收较差，此作用未能在短时间内提高胆汁和血浆中芍药苷浓度。
