Department of Biology, Faculty of Science, University of Hacettepe, Ankara, Turkey.
Food Chem Toxicol. 2013 Jan;51:123-36. doi: 10.1016/j.fct.2012.09.010. Epub 2012 Sep 25.
The present study is to investigate the effects of in utero di-n-hexyl phthalate (DHP) and dicyclohexyl phthalate exposure (DCHP) on the development of male reproductive tract at prepubertal, pubertal and adult stages. Pregnant rats were exposed to DHP and DCHP at doses of 0, 20, 100 and 500mg/kg/day, by gavage, on gestational days (GD) 6-19. Testosterone (T) levels of prepubertal rats diminished at high dose DHP and middle dose DCHP groups. MIS/AMH levels elevated in DHP and DCHP groups. T levels of pubertal rats decreased in low and high dose DHP and DCHP groups. Inhibin B levels of adult rats diminished in DCHP groups. Atrophic and amorphous tubules, spermatogenic cell debris, apoptotic cells, adherent tubules, Sertoli cell vacuolisation, prostatic atrophic tubules and prostatic intraepithelial neoplasia (PIN) were observed in the reproductive organs of treated animals at all developmental stages. There was an increase in immunoexpression of MIS/AMH in testes of treated rats. There were no changes in sperm head count but percentages of abnormal sperms increased. The diameters of seminiferous and epididymal tubules in treatment groups were significantly lower. This study shows that DHP and DCHP may have antiandrogenic effects on male reproductive development before and after birth.
本研究旨在探讨孕期暴露于邻苯二甲酸二己酯（DHP）和邻苯二甲酸二环己酯（DCHP）对雄性生殖管道在青春期前、青春期和成年期发育的影响。妊娠大鼠在妊娠第 6-19 天通过灌胃暴露于 0、20、100 和 500mg/kg/天的 DHP 和 DCHP。青春期前大鼠的睾丸酮（T）水平在高剂量 DHP 和中剂量 DCHP 组降低。DHP 和 DCHP 组中 MIS/AMH 水平升高。青春期大鼠的 T 水平在低剂量和高剂量 DHP 和 DCHP 组中降低。成年大鼠的抑制素 B 水平在 DCHP 组中降低。在所有发育阶段，处理动物的生殖器官中观察到萎缩和非晶形小管、生殖细胞碎片、凋亡细胞、黏附小管、支持细胞空泡化、前列腺萎缩小管和前列腺上皮内瘤变（PIN）。在处理大鼠的睾丸中，MIS/AMH 的免疫表达增加。精子头计数没有变化，但异常精子的百分比增加。治疗组的精母细胞和附睾小管的直径明显降低。本研究表明，DHP 和 DCHP 可能对出生前后雄性生殖发育具有抗雄激素作用。
