HMG1蛋白乙酰化形式与非乙酰化形式某些特性之间的差异。

Differences between some properties of acetylated and nonacetylated forms of HMG1 protein.

作者信息

Dimov S I, Alexandrova E A, Beltchev B G

机构信息

Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia.

出版信息

Biochem Biophys Res Commun. 1990 Jan 30;166(2):819-26. doi: 10.1016/0006-291x(90)90883-o.

DOI:10.1016/0006-291x(90)90883-o

PMID:2302240

Abstract

There are data suggesting that HMG1 protein may be involved in DNA replication. Recently we have found that only the acetylated form of the protein generates tetramers, stimulates the activity of DNA polymerase alpha (EC 2.7.7.7) (with activated DNA as a template) and forms a specific complex with it. This paper compares some properties of the acetylated and nonacetylated forms of HMG1 protein and shows that it is only the acetylated form which serves as a histone assembly factor, increases the melting temperature of poly d[(A-T)] and stimulates the activity of DNA polymerase alpha when histone H1-depleted chromatin is used as a template.

摘要

有数据表明，HMG1蛋白可能参与DNA复制。最近我们发现，只有该蛋白的乙酰化形式能生成四聚体，刺激DNA聚合酶α（EC 2.7.7.7）的活性（以活化的DNA为模板），并与之形成特定复合物。本文比较了HMG1蛋白乙酰化和非乙酰化形式的一些特性，结果表明，只有乙酰化形式可作为组蛋白组装因子，提高聚d[(A-T)]的解链温度，并在以缺乏组蛋白H1的染色质为模板时刺激DNA聚合酶α的活性。