CRUK/MRC Gray Institute for Radiation Oncology and Biology, Department of Oncology, University of Oxford, OX3-7DQ, Oxford, UK.
J Magn Reson Imaging. 2013 Jun;37(6):1499-504. doi: 10.1002/jmri.23829. Epub 2012 Sep 28.
To describe a combination of techniques using the excellent volumetric capacities of magnetic resonance imaging (MRI) while avoiding anesthesia and maintaining high-throughput capability for tumor volume measurement in the awake mouse. This approach presents an alternative to calipers which, although cheap, fast, and easy to use, introduce many biases for tumor volume estimation.
The murine CaNT subcutaneous xenograft model was used. A quiet and modestly T2-weighted spin-echo scan was acquired at 4.7T (TE = 15 msec, TR = 1100 msec, 0.5 mm isotropic resolution) while the awake mouse was held by hand in the magnet. This method was compared to standard MR in the anesthetized mouse and caliper measurements.
The combination of techniques used allows rapid, accurate, and reproducible measurement of subcutaneous tumor volumes in awake mice. It is less sensitive to both intra- and interoperator-derived biases and avoids confounds from the compliance of the fat and skin around the tumor, as well as from the tumor itself. Moreover, the data remain available for retrieval and scrutiny and reanalysis.
Rapid, accurate, and precise tumor volumetry can be performed in the awake mouse by handheld positioned MR.
描述一种结合使用磁共振成像（MRI）优异容积能力的技术组合，同时避免麻醉并保持在清醒小鼠中进行肿瘤体积测量的高通量能力。这种方法替代了卡尺，虽然卡尺便宜、快速且易于使用，但在肿瘤体积估计方面引入了许多偏差。
使用了鼠 CaNT 皮下异种移植模型。在 4.7T 时采集安静且适度 T2 加权自旋回波扫描（TE = 15 msec，TR = 1100 msec，0.5 毫米各向同性分辨率），同时用手将清醒的小鼠固定在磁体中。将这种方法与麻醉小鼠中的标准 MRI 和卡尺测量进行了比较。
所使用的技术组合允许快速、准确和可重复地测量清醒小鼠的皮下肿瘤体积。它对操作者内和操作者间偏差的敏感性较低，并且避免了肿瘤周围脂肪和皮肤的顺应性以及肿瘤本身带来的混杂因素。此外，数据仍然可用于检索、审查和重新分析。
通过手持定位 MRI 可以在清醒的小鼠中进行快速、准确和精确的肿瘤体积测量。
