AEG-1 在人 T 细胞淋巴瘤中的表达增加了进展的风险。

Expression of AEG-1 in human T-cell lymphoma enhances the risk of progression.

机构信息

Department of Oncology, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan 450052, PR China.

出版信息

Oncol Rep. 2012 Dec;28(6):2107-14. doi: 10.3892/or.2012.2055. Epub 2012 Sep 26.

DOI:10.3892/or.2012.2055

Abstract

The aim of this study was to examine the expression and role of astrocyte elevated gene-1 (AEG-1) in biological processes of T-cell non-Hodgkin's lymphoma (T-NHL). AEG-1 expression in T-NHL patients was characterized with immunohistochemistry. The expression of AEG-1, survivin, Bcl-2 and Bax in Jurkat and Hut-78 cells was detected by real-time PCR and western blotting. Cell proliferation, cell cycle and apoptosis were measured by MTT and flow cytometry. MMP-2/-9 activity was detected by gelatin zymography. Of the studied tumors, 104 (80.62%) exhibited cytoplasmic AEG-1 immunostaining. AEG-1-siRNA in Jurkat and Hut-78 cells suppressed cell proliferation and induced cell apoptosis, inhibited survivin and Bcl-2/Bax protein expression as well as MMP-2/-9 activity. Downregulation of AEG-1 using siRNA could provide a potential approach for gene therapy against T-NHL, and the antitumor effects may be associated with inhibition of survivin and Bcl-2/Bax protein expression and MMP-2/-9 activity.

摘要

本研究旨在探讨星形细胞上调基因-1（AEG-1）在 T 细胞非霍奇金淋巴瘤（T-NHL）生物学过程中的表达和作用。采用免疫组织化学方法检测 T-NHL 患者中 AEG-1 的表达。采用实时 PCR 和 Western blot 检测 Jurkat 和 Hut-78 细胞中 AEG-1、survivin、Bcl-2 和 Bax 的表达。通过 MTT 和流式细胞术检测细胞增殖、细胞周期和细胞凋亡。通过明胶酶谱法检测 MMP-2/-9 活性。在研究的肿瘤中，104 例（80.62%）表现出细胞质 AEG-1 免疫染色。Jurkat 和 Hut-78 细胞中的 AEG-1-siRNA 抑制细胞增殖并诱导细胞凋亡，抑制 survivin 和 Bcl-2/Bax 蛋白表达以及 MMP-2/-9 活性。用 siRNA 下调 AEG-1 可能为 T-NHL 的基因治疗提供一种潜在的方法，抗肿瘤作用可能与抑制 survivin 和 Bcl-2/Bax 蛋白表达以及 MMP-2/-9 活性有关。