从蓝藻微囊藻 Ku2 中部分纯化和鉴定一种弹性蛋白酶抑制性脂质衍生物。
Partial Purification and Characterization of an Elastase-inhibitory Lipid Derivative from a Cyanobacterium, Microcystis Ku2.
机构信息
Department of Biological Sciences, Rani Durgavati University, Jabalpur, 482001 India.
出版信息
Indian J Microbiol. 2011 Oct;51(4):496-500. doi: 10.1007/s12088-011-0120-7. Epub 2011 Jan 26.
Abstract
A unicyanobacterial isolate of cyanobacterium, identified as Microcystis Ku2, produced a mammalian elastase-inhibitory lipid derivative. Protease inhibitors in cyanobacteria are unequivocally peptides. Since this metabolite appeared in lipid phase, we worked on a hypothesis that whether metabolite other than peptides could be responsible for the characteristic inhibition. It was purified by saponification and reverse phase column chromatography. The resulting compound was tentatively characterized as a glycolipid with structure of sulfated di-pentose derivative of ceramide (MW = 956 Da). The apparent IC(50) for elastase was 1.3 μM.
摘要
一株蓝细菌（鉴定为微囊藻 Ku2）产生了一种抑制哺乳动物弹性蛋白酶的脂类衍生物。蓝细菌中的蛋白酶抑制剂无疑是肽类。由于这种代谢物出现在脂相中，我们假设除肽类以外的代谢物是否可能对特征性抑制负责。它通过皂化和反相柱层析进行纯化。得到的化合物被初步鉴定为神经酰胺的二戊糖磺酸酯衍生物（MW=956 Da）的糖脂。弹性蛋白酶的表观 IC50 为 1.3 μM。
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