Institute of Science, Nirma University, Sarkhej-Gandhinagar Highway, Ahmedabad, 382481 Gujarat India.
Indian J Microbiol. 2011 Oct;51(4):521-7. doi: 10.1007/s12088-011-0149-7. Epub 2011 Feb 26.
Since past three decades new discoveries in plant genetic engineering have shown remarkable potentials for crop improvement. Agrobacterium Ti plasmid based DNA transfer is no longer the only efficient way of introducing agronomically important genes into plants. Recent studies have explored a novel plant genetic engineering tool, Rhizobia sp., as an alternative to Agrobacterium, thereby expanding the choice of bacterial species in agricultural plant biotechnology. Rhizobia sp. serve as an open license source with no major restrictions in plant biotechnology and help broaden the spectrum for plant biotechnologists with respect to the use of gene transfer vehicles in plants. New efficient transgenic plants can be produced by transferring genes of interest using binary vector carrying Rhizobia sp. Studies focusing on the interactions of Rhizobia sp. with their hosts, for stable and transient transformation and expression of genes, could help in the development of an adequate gene transfer vehicle. Along with being biologically beneficial, it may also bring a new means for fast economic development of transgenic plants, thus giving rise to a new era in plant biotechnology, viz. "Rhizobia mediated transformation technology."
三十年来,植物基因工程的新发现显示出了改良作物的巨大潜力。基于根瘤农杆菌 Ti 质粒的 DNA 转移已不再是将具有重要农艺学意义的基因导入植物的唯一有效途径。最近的研究探索了一种新型的植物基因工程工具——根瘤菌,作为根瘤农杆菌的替代物,从而扩大了农业植物生物技术中细菌种类的选择。根瘤菌作为一个开放许可的来源,在植物生物技术中没有重大限制,有助于拓宽植物生物技术工作者在植物中使用基因转移载体的范围。通过使用携带根瘤菌的二元载体转移感兴趣的基因,可以生产出高效的转基因植物。专注于根瘤菌与其宿主之间相互作用的研究,对于基因的稳定和瞬时转化和表达,有助于开发出合适的基因转移载体。这种方法不仅具有生物学上的益处,也为转基因植物的快速经济发展带来了新的手段,从而开启了植物生物技术的新时代,即“根瘤菌介导的转化技术”。