Tzfira Tzvi, Citovsky Vitaly
Department of Molecular, Cellular and Developmental Biology, The University of Michigan, Ann Arbor, MI 48109, USA.
Curr Opin Biotechnol. 2006 Apr;17(2):147-54. doi: 10.1016/j.copbio.2006.01.009. Epub 2006 Feb 3.
Agrobacterium-mediated genetic transformation is the dominant technology used for the production of genetically modified transgenic plants. Extensive research aimed at understanding and improving the molecular machinery of Agrobacterium responsible for the generation and transport of the bacterial DNA into the host cell has resulted in the establishment of many recombinant Agrobacterium strains, plasmids and technologies currently used for the successful transformation of numerous plant species. Unlike the role of bacterial proteins, the role of host factors in the transformation process has remained obscure for nearly a century of Agrobacterium research, and only recently have we begun to understand how Agrobacterium hijacks host factors and cellular processes during the transformation process. The identification of such factors and studies of these processes hold great promise for the future of plant biotechnology and plant genetic engineering, as they might help in the development of conceptually new techniques and approaches needed today to expand the host range of Agrobacterium and to control the transformation process and its outcome during the production of transgenic plants.
农杆菌介导的遗传转化是用于生产转基因植物的主导技术。旨在理解和改进农杆菌负责将细菌DNA生成并转运到宿主细胞中的分子机制的广泛研究，已促成了许多重组农杆菌菌株、质粒和技术的建立，这些目前被用于成功转化众多植物物种。与细菌蛋白的作用不同，在近一个世纪的农杆菌研究中，宿主因子在转化过程中的作用一直模糊不清，直到最近我们才开始了解农杆菌在转化过程中是如何劫持宿主因子和细胞过程的。鉴定这些因子并研究这些过程对植物生物技术和植物基因工程的未来有着巨大的前景，因为它们可能有助于开发当今所需的概念上新的技术和方法，以扩大农杆菌的宿主范围，并在转基因植物生产过程中控制转化过程及其结果。
