Kikuchi Y, Kita T, Nagata I
Department of Obstetrics and Gynecology, National Defense Medical College, Saitama, Japan.
Cancer Immunol Immunother. 1990;30(6):374-6. doi: 10.1007/BF01786888.
The present study was designed to explore the effects of opioid peptides on the lytic activity of spleen cells from intact nude mice or nude mice bearing human ovarian cancer cells (KF). When the spleen cells from intact nude mice were incubated with various concentrations of opioid peptides, the ability of the spleen cells to lyse the KF cells was significantly stimulated between 0.05 nM and 50 nM concentrations of all opioid peptides used in this study. The degree of stimulation was most marked at 5 nM opioid peptides and the most marked stimulatory effect was obtained by alpha-endorphin. On the other hand, the lytic activity of spleen cells from nude mice challenged with the KF cells was about two-fold higher than that of intact nude mice, suggesting that spleen cells from nude mice challenged with KF cells have KF-cell-specific cytotoxicity. Even if the spleen cells were incubated with any concentration of alpha-endorphin or [Met]enkephalin indicated, the lytic activity remained unchanged. In contrast, only beta-endorphin resulted in a significant increase of the lytic activity between 0.5 nM and 50 nM. These results suggest that opioid peptides play a crucial role in immune surveillance mechanisms.
本研究旨在探讨阿片肽对完整裸鼠或荷人卵巢癌细胞（KF）裸鼠脾细胞溶解活性的影响。当将完整裸鼠的脾细胞与不同浓度的阿片肽一起孵育时，在本研究中所用的所有阿片肽浓度为0.05 nM至50 nM之间，脾细胞溶解KF细胞的能力均受到显著刺激。刺激程度在5 nM阿片肽时最为明显，且α-内啡肽产生的刺激作用最为显著。另一方面，用KF细胞攻击的裸鼠脾细胞的溶解活性比完整裸鼠的高约两倍，这表明用KF细胞攻击的裸鼠脾细胞具有KF细胞特异性细胞毒性。即使脾细胞与所示的任何浓度的α-内啡肽或[甲硫氨酸]脑啡肽一起孵育，其溶解活性仍保持不变。相反，仅β-内啡肽在0.5 nM至50 nM之间导致溶解活性显著增加。这些结果表明阿片肽在免疫监视机制中起关键作用。
