Iwasaki K, Kikuchi H, Miyatake S, Oda Y, Yamashita J, Yamasaki T
Department of Neurosurgery, Kyoto University Medical School, Japan.
Cell Immunol. 1990 Mar;126(1):164-75. doi: 10.1016/0008-8749(90)90309-f.
The clonal analysis in the ultrastructure of tumor-lymphocyte interaction was carried out in order to investigate the precise mechanism responsible for CTL-mediated cytolysis of tumor cells. A glioma-derived cell line (GI-1) and autologous tumor-specific cytotoxic T lymphocyte (CTL) clones were established. The CTL lines were composed of the morphologically homogeneous lymphocytes with intracytoplasmic electron-dense secretory granules. After the stimulation by GI-1, the size of the CTLs increased, and the intracytoplasmic organellas were developed. It was noted that the intracytoplasmic secretory granules markedly increased in number and size, and many of them exhibited an "immature" appearance. On the other hand, the tumor cells underwent a progressive degeneration. In contrast, the stimulation by other antigens caused only small morphological changes in the CTLs. It is suggested, therefore, that the secretory function of tumor-specific CTLs is activated by the stimulation of the specific antigen, and that soluble factors in the secretory granules in the CTLs may be closely associated with the mechanism of target cell lysis.
为了研究细胞毒性T淋巴细胞（CTL）介导的肿瘤细胞溶解的确切机制，对肿瘤-淋巴细胞相互作用的超微结构进行了克隆分析。建立了一种胶质瘤衍生细胞系（GI-1）和自体肿瘤特异性细胞毒性T淋巴细胞（CTL）克隆。CTL系由形态学上均一的淋巴细胞组成，胞质内有电子致密的分泌颗粒。经GI-1刺激后，CTL的大小增加，胞质内细胞器发育。值得注意的是，胞质内分泌颗粒的数量和大小明显增加，其中许多呈现“不成熟”外观。另一方面，肿瘤细胞发生进行性退变。相比之下，其他抗原刺激仅引起CTL微小的形态学变化。因此，提示肿瘤特异性CTL的分泌功能被特异性抗原刺激激活，CTL分泌颗粒中的可溶性因子可能与靶细胞溶解机制密切相关。
