Inhalation Toxicology Center, Jeonbuk Department of Non-human Primate, Korea Institute of Toxicology, Jeonbuk, 580-185, Korea; College of Human Ecology, Seoul National University, Seoul, 151-742, Korea.
Phytother Res. 2013 Aug;27(8):1200-5. doi: 10.1002/ptr.4847. Epub 2012 Oct 2.
Ginger has long been used worldwide as a spice, seasoning, and wine and is also used as a traditional medicine. There have been no previous studies of the potential beneficial effects of the ginger constituent 12-dehydrogingerdione (12-DHGD). We investigated the anti-inflammatory effect of 12-DHGD on lipopolysaccharide (LPS)-stimulated Raw 264.7 cells. The cytotoxicity of 12-DHGD was measured using the MTT assay, and production of prostaglandin E2 (PGE2 ) and the inflammatory cytokines interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α was measured by ELISA. Production of nitric oxide (NO) was measured using Griess reagent and expression of cyclooxygenase-2 (COX-2) and inducible NO (iNOS) enzymes was assessed by reverse transcriptase-polymerase chain reaction. Treatment of Raw 264.7 cells with 12-DHGD significantly inhibited LPS-stimulated production of NO (at 12-DHGD concentrations of 150 and 200 ng/ml), IL-6 (at 50, 100, 150, and 200 ng/ml), and PGE2 (at 200 ng/ml). Consistent with the effects on NO and PGE2 production, 12-DHGD treatment also inhibited the LPS-stimulated increase in iNOS and COX-2 mRNA levels. However, 12-DHGD did not affect production of IL-1β or TNF-α in response to LPS. 12-DHGD, a constituent of ginger, is a potent inhibitor of proinflammatory mediator production in Raw 264.7 macrophage cells.
姜在全世界范围内一直被用作香料、调味料和酒类，也被用作传统药物。此前尚未研究过生姜成分 12-去氢姜二酮（12-DHGD）的潜在有益作用。我们研究了 12-DHGD 对脂多糖（LPS）刺激的 Raw 264.7 细胞的抗炎作用。用 MTT 法测定 12-DHGD 的细胞毒性，用 ELISA 法测定前列腺素 E2（PGE2）和炎症细胞因子白细胞介素（IL）-1β、IL-6 和肿瘤坏死因子（TNF）-α的产生。用 Griess 试剂测定一氧化氮（NO）的产生，用逆转录-聚合酶链反应评估环氧化酶-2（COX-2）和诱导型一氧化氮合酶（iNOS）酶的表达。用 12-DHGD 处理 Raw 264.7 细胞可显著抑制 LPS 刺激的 NO（12-DHGD 浓度为 150 和 200ng/ml）、IL-6（50、100、150 和 200ng/ml）和 PGE2（200ng/ml）的产生。与对 NO 和 PGE2 产生的作用一致，12-DHGD 处理还抑制了 LPS 刺激的 iNOS 和 COX-2 mRNA 水平的增加。然而，12-DHGD 不影响 LPS 刺激产生的 IL-1β或 TNF-α。姜的一种成分 12-DHGD 是 Raw 264.7 巨噬细胞中促炎介质产生的有效抑制剂。
