Department of Oncology, Vejle Hospital, Denmark.
APMIS. 2013 Feb;121(2):127-38. doi: 10.1111/j.1600-0463.2012.02949.x. Epub 2012 Aug 17.
The aim of this study was to describe the dynamics of HIF-1α, GLUT-1, Bcl-2 and Ki-67 during chemoradiotherapy (CRT) of rectal cancer, and to investigate the fluctuation of these biomarkers in relation to pathological response to CRT. The study included 86 patients with rectal adenocarcinoma receiving preoperative CRT (>50.4 Gy and Uracil/Tegafur). Immunohistological expressions of HIF-1α, GLUT-1, Bcl-2 and Ki-67 were investigated in biopsies taken before treatment, after 2, 4 and 6 weeks of CRT and in specimens from the operation. Decreasing expressions of HIF-1α, Bcl-2 and Ki-67 were observed during CRT, whereas GLUT-1 overall was unchanged. No significant changes of the markers were observed in the interval between CRT and surgery. A significant association was observed between the presence of residual carcinoma after 6 weeks of treatment and pathological response to CRT, but no association was seen between the fluctuations of any of the markers and response to CRT. This unique material containing specimens before, after and during CRT for rectal cancer demonstrated biological dynamics in HIF-1α, Bcl-2 and Ki-67, but not GLUT-1, expression during CRT, and a significant association was seen between the presence of residual carcinoma after 6 weeks of treatment and pathological response to CRT.
本研究旨在描述直肠腺癌放化疗过程中 HIF-1α、GLUT-1、Bcl-2 和 Ki-67 的动态变化，并探讨这些生物标志物的波动与 CRT 病理反应的关系。该研究纳入了 86 例接受术前 CRT（>50.4 Gy 和 Uracil/Tegafur）的直肠腺癌患者。在治疗前、治疗后 2、4 和 6 周以及手术标本中，对 HIF-1α、GLUT-1、Bcl-2 和 Ki-67 的免疫组织化学表达进行了研究。在 CRT 过程中观察到 HIF-1α、Bcl-2 和 Ki-67 的表达逐渐降低，而 GLUT-1 总体上没有变化。在 CRT 和手术之间的间隔期间，标志物没有明显变化。治疗 6 周后残留癌的存在与 CRT 的病理反应之间存在显著相关性，但任何标志物的波动与 CRT 的反应之间均无相关性。本研究独特的材料包含了直肠癌治疗前、治疗中和治疗后的标本，结果显示在 CRT 过程中 HIF-1α、Bcl-2 和 Ki-67 的表达存在生物学动态变化，但 GLUT-1 没有变化，治疗 6 周后残留癌的存在与 CRT 的病理反应之间存在显著相关性。
