Department of Experimental and Clinical Medicine and Pharmacology, Section of Pharmacology, University of Messina, Italy.
J Clin Periodontol. 2013 Jan;40(1):26-32. doi: 10.1111/jcpe.12010. Epub 2012 Oct 3.
Adenosine receptors modulate inflammation in periodontal tissues. No data are available regarding the effects of adenosine A(2A) receptor stimulation in experimental periodontitis (EPD). The aim of this study was to investigate the effects of polynucleotides (also known as polydeoxyribonucleotide, PDRN), a ligand of A(2A) receptor, in EPD in rats.
EPD was induced ligating the cervix of the lower left first molar. Sham-EPD had no ligature. After 7 days, EPD animals were randomized to a daily treatment with vehicle gel or 0.75% PDRN gel or PDRN gel with a specific A(2A) antagonist (DMPX). Treatments lasted 7 days. Animals were then euthanized and the periodontium and surrounding gingival tissue were excised for histological evaluation and bio-molecular analysis of inflammatory (p-JNK, p-ERK, TNF-α, IL-6, HMGB-1) and apoptotic proteins (BAX and Bcl-2).
Vehicle-treated EPD rats showed severe inflammatory infiltrate in both gingival and periodontal ligament, as well as an enhanced expression of p-JNK, p-ERK, TNF-α, IL-6, HMGB-1 and BAX and a reduction in Bcl-2. PDRN gel restored the histological features, blunted inflammatory and apoptotic proteins expression and preserved Bcl-2 expression. DMPX abrogated PDRN positive effects.
Our data suggest that adenosine receptor stimulation by PDRN might represent a new therapeutic strategy for periodontitis.
腺苷受体可调节牙周组织的炎症。关于腺苷 A（2A）受体刺激在实验性牙周炎（EPD）中的作用尚无数据。本研究旨在探讨多核苷酸（也称为聚脱氧核糖核苷酸，PDRN），即 A（2A）受体配体，在大鼠 EPD 中的作用。
结扎左下第一磨牙的颈圈可诱导 EPD。 Sham-EPD 没有结扎。7 天后，EPD 动物随机分为每天接受 vehicle 凝胶或 0.75% PDRN 凝胶或 PDRN 凝胶加特定 A（2A）拮抗剂（DMPX）治疗。治疗持续 7 天。然后处死动物，切除牙周组织和周围牙龈组织，进行组织学评估和炎症（p-JNK、p-ERK、TNF-α、IL-6、HMGB-1）和凋亡蛋白（BAX 和 Bcl-2）的生物分子分析。
用 vehicle 处理的 EPD 大鼠在牙龈和牙周韧带中均显示出严重的炎症浸润，以及 p-JNK、p-ERK、TNF-α、IL-6、HMGB-1 和 BAX 的表达增强，而 Bcl-2 的表达减少。PDRN 凝胶恢复了组织学特征，减弱了炎症和凋亡蛋白的表达，并保留了 Bcl-2 的表达。DMPX 消除了 PDRN 的积极作用。
我们的数据表明，PDRN 对腺苷受体的刺激可能代表了牙周炎的一种新的治疗策略。
