Department of Biology, Faculty of Science, J.E. Purkinje University in Ústí nad Labem, České mládeže 8, 400 96 Ústí nad Labem, Czech Republic.
Microsc Res Tech. 2013 Jan;76(1):28-35. doi: 10.1002/jemt.22131. Epub 2012 Oct 4.
Scanning electron microscopy and atomic force microscopy were used to study the silk spinning apparatus and silks of Harpactea rubicunda spiders. Three types of silk secretions that are produced by three kinds of silk spinning glands (ampullate, piriform, and pseudaciniform) and released through three types of spigots, were confirmed for both adult and juvenile spiders. Silk secretions for the construction of spider webs for shelter or retreat are produced by the pseudaciniform silk glands. Silk secretions that are released from spigots in the course of web construction are not processed by the legs during the subsequent process of hardening. Pairs of nanofibril bundles seemed to be part of the basic microarchitecture of the web silk fibers as revealed by AFM. These fiber bundles frequently not only overlap one another, but occasionally also interweave. This structural variability may strengthen the spider web. High-resolution AFM scans of individual nanofibrils show a distinctly segmented nanostructure. Each globular segment is ∼30-40 nm long along the longitudinal axis of the fiber, and resembles a nanosegment of artificial fibroin described by Perez-Rigueiro et al. (2007).
扫描电子显微镜和原子力显微镜被用于研究 Rubicunda 捕鸟蛛的丝腺和丝。确定了成年和幼年蜘蛛的三种丝腺（壶腹状腺、瓶状腺和拟晶质腺）产生的三种丝分泌物，通过三种喷丝头释放。用于构建庇护所或撤退用蜘蛛网的丝分泌物由拟晶质腺丝腺产生。在网构建过程中从喷丝头释放的丝分泌物在随后的硬化过程中不会被腿处理。通过原子力显微镜观察到，成对的纳米原纤维束似乎是蛛网丝纤维基本微观结构的一部分。这些纤维束不仅经常相互重叠，而且偶尔还相互交织。这种结构的可变性可能增强了蜘蛛网。对单个纳米纤维的高分辨率 AFM 扫描显示出明显的分段纳米结构。每个球形段沿纤维的纵轴约 30-40nm 长，类似于 Perez-Rigueiro 等人描述的人工丝素的纳米段（2007）。
