Dipartimento Farmaco-Chimico, Facoltà di Farmacia, Università degli Studi di Messina, Messina, Italy.
J Sep Sci. 2013 Jan;36(1):212-8. doi: 10.1002/jssc.201200592. Epub 2012 Oct 4.
The present manuscript reports a comparative study between conventional and split-flow comprehensive GC×GC-MS. Conventional GC×GC-MS was performed by using a single GC oven, and with a widely employed apolar (30 m × 0.25 mm id)-polar (1 m × 0.10 mm id) column combination. Split-flow GC×GC-MS experiments were carried out by using the same first and second analytical columns, and by diverting part of the primary-capillary flow to waste, via a waste line. The latter was located at the conjunction point between the two dimensions. The measurement of fundamental gas chromatography parameters (efficiency, resolution), in both dimensions and under different experimental conditions, enabled the attainment of detailed information related to GC×GC-MS method optimization. Finally, the results derived from the present investigation highlight an important GC×GC issue, namely the efficiency of band reinjection onto the second-dimension capillary.
本手稿报告了常规和分流全二维气相色谱-飞行时间质谱（GC×GC-TOFMS）的对比研究。常规 GC×GC-TOFMS 采用单 GC 炉进行，采用广泛应用的非极性（30 m×0.25 mm id）-极性（1 m×0.10 mm id）柱组合。分流流 GC×GC-TOFMS 实验使用相同的第一和第二分析柱，并通过废气管将一部分初级毛细物流分流至废物，废气管位于二维交界处。测量两个维度和不同实验条件下的基本气相色谱参数（效率、分辨率），可获得与 GC×GC-TOFMS 方法优化相关的详细信息。最后，本研究的结果突出了一个重要的 GC×GC 问题，即带重新注入到第二维毛细管的效率。
