Lane Randy, See Seong S
Department of Natural Sciences, Albany State University, Albany, GA 31705, USA.
J Nanosci Nanotechnol. 2012 Sep;12(9):7395-400. doi: 10.1166/jnn.2012.6582.
Attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) is used to find the structural differences between cancerous breast cells (MCF-7 line) and normal breast cells (MCF-12F line). Gold nanoparticles were prepared and the hydrodynamic diameter of the gold nanoparticles found to be 38.45 nm. The Gold nanoparticles were exposed to both MCF-7 and MCF-12F cells from lower to higher concentrations. Spectroscopic studies founds nanoparticles were within the cells, and increasing the nanoparticles concentration inside the cells also resulted in sharper IR peaks as a result of localized surface Plasmon resonance. Asymmetric and symmetric stretching and bending vibrations between phosphate, COO-, CH2 groups were found to give negative shifts in wavenumbers and a decrease in peak intensities when going from noncancerous to cancerous cells. Cellular proteins produced peak assignments at the 1542 and 1644 cm(-1) wavenumbers which were attributed to the amide I and amide II bands of the polypeptide bond of proteins. Significant changes were found in the peak intensities between the cell lines in the spectrum range from 2854-2956 cm(-1). Results show that the concentration range of gold nanoparticles used in this research showed no significant changes in cell viability in either cell line. Therefore, we believe ATR-FTIR and gold nanotechnology can be at the forefront of cancer diagnosis for some time to come.
衰减全反射傅里叶变换红外光谱法（ATR-FTIR）用于寻找癌性乳腺细胞（MCF-7系）和正常乳腺细胞（MCF-12F系）之间的结构差异。制备了金纳米颗粒，发现其流体动力学直径为38.45纳米。将金纳米颗粒以从低到高的浓度暴露于MCF-7和MCF-12F细胞。光谱研究发现纳米颗粒存在于细胞内，并且由于局域表面等离子体共振，细胞内纳米颗粒浓度的增加也导致红外峰更尖锐。发现从非癌细胞到癌细胞时，磷酸盐、COO-、CH2基团之间的不对称和对称伸缩及弯曲振动导致波数出现负向位移且峰强度降低。细胞蛋白质在1542和1644 cm(-1)波数处产生峰归属，这归因于蛋白质多肽键的酰胺I和酰胺II带。在2854 - 2956 cm(-1)光谱范围内，细胞系之间的峰强度存在显著变化。结果表明，本研究中使用的金纳米颗粒浓度范围在两种细胞系中均未显示细胞活力有显著变化。因此，我们相信在未来一段时间内，ATR-FTIR和金纳米技术能够处于癌症诊断的前沿。
