Arbuzova V S, Badaeva E D, Efremova T T, Osadchaia T S, Trubacheva N V, Dobrovol'skaia O B
Genetika. 2012 Aug;48(8):926-33.
The chromosome composition of the blue-grain line i:S29Ba of the cultivar Saratovskaya 29 was identified by cytological, GISH, and microsatellite analyses and C-banding. It was found that common wheat chromosome 4B of the cultivar Saratovskaya 29 was substituted with the Agropyron elongatum Host. chromosome carrying the gene for blue grain (s:S294Ag(4B)) during the construction of this nearly isogenic line. The blue-grain line was tested for productivity. The substitution of total chromosome 4B of the cultivar Saratovskaya 29 by Ag. elongatum chromosome 4 did not significantly affect the spike productivity parameters and grain quality with the exception of spike length (plus effect), spike density, and vitreousness (minus effects). The blue-grain line with s:S294Ag(4B) can be used in further studies associated with chromosome engineering in cereals and wheat breeding.
通过细胞学、基因组原位杂交（GISH）、微卫星分析和C带分析，对品种萨拉托夫斯卡娅29（Saratovskaya 29）的蓝粒品系i:S29Ba的染色体组成进行了鉴定。结果发现，在构建这个近等基因系的过程中，萨拉托夫斯卡娅29品种的普通小麦4B染色体被携带蓝粒基因的长穗偃麦草（Agropyron elongatum Host.）染色体所取代（s:S294Ag(4B)）。对该蓝粒品系进行了生产力测试。用长穗偃麦草4号染色体取代萨拉托夫斯卡娅29品种的整个4B染色体，除了穗长（正效应）、穗密度和玻璃质化程度（负效应）外，对穗生产力参数和籽粒品质没有显著影响。携带s:S294Ag(4B)的蓝粒品系可用于与谷物染色体工程和小麦育种相关的进一步研究。
